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Overview

Uniprot IDA0A0G2JTY6
Protein NameG-rich sequence factor 1
Gene NameGrsf1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
241 VGSHKGKKMVSSPTT

Function

Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences. Involved in the degradosome-mediated decay of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules. Acts by unwinding G-quadruplex RNA structures in MT-ncRNA, thus facilitating their degradation by the degradosome. G-quadruplexes (G4) are non-canonical 4 stranded structures formed by transcripts from the light strand of mtDNA

Protein Sequence

10 MLRDGGLMMC 20 FVFGAIFSET 30 ESKTTYLEDL 40 PPLPEYELSP 50 SKLGEEVDDV 60 YLIRAQGLPW 70 SCTVEDVLSF 80 FSDCRIRNSE 90 NGIHFLLNRD 100 GKRRGDALIE 110 MESEQDVQKA 120 LEKHRMYMGQ 130 RYVEVYEINN 140 EDVDALMKSL 150 QVKPTPALSD 160 GVVRLRGLPY 170 SCNEKDIVDF 180 FAGLNIVDIT 190 FVMDYRGRRK 200 TGEAYVQFEE 210 PEMANQALLK 220 HREEIGNRYI 230 EIFPSRRNEV 240 RTHVGSHKGK 250 KMVSSPTTKY 260 ITEPEVVFEE 270 HEVNEDARPM 280 TAFESEKEIE 290 LPKEMSDKLA 300 EAVDFGAVPS 310 LHFVHMRGLP 320 FQANAQDIIN 330 FFAPLKPVRI 340 TMEYSSSGKA 350 TGEADVHFDT 360 HEDAVAAMLK 370 DRSHVQHRYI 380 ELFLNSCPKG K

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Molecular Function GO:0003723 RNA binding
Biological Process GO:0006397 mRNA processing
Biological Process GO:0008033 tRNA processing

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.