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Overview

Uniprot IDA0A0G2JWK2
Protein NameMethyl-CpG-binding protein 2
Gene NameMecp2
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
215 SPGKLLVKMPFQASP
22 EKPLKFKKVKKDKKE
24 PLKFKKVKKDKKEDK

Function

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC)

Protein Sequence

10 REEKSEDQDL 20 QGLKEKPLKF 30 KKVKKDKKED 40 KEGKHEPLQP 50 SAHHSAEPAE 60 AGKAETSESS 70 GSAPAVPEAS 80 ASPKQRRSII 90 RDRGPMYDDP 100 TLPEGWTRKL 110 KQRKSGRSAG 120 KYDVYLINPQ 130 GKAFRSKVEL 140 IAYFEKVGDT 150 SLDPNDFDFT 160 VTGRGSPSRR 170 EQKPPKKPKS 180 PKAPGTGRGR 190 GRPKGSGTGR 200 PKAAASEGVQ 210 VKRVLEKSPG 220 KLLVKMPFQA 230 SPGGKGEGGG 240 ATTSAQVMVI 250 KRPGRKRKAE 260 ADPQAIPKKR 270 GRKPGSVVAA 280 AAAEAKKKAV 290 KESSIRSVQE 300 TVLPIKKRKT 310 RETVSIEVKE 320 VVKPLLVSTL 330 GEKSGKGLKT 340 CKSPGRKSKE 350 SSPKGRSSSA 360 SSPPKKEHHH 370 HHHHAESPKA 380 PMPLLPPPPP 390 PEPQSSEDPI 400 SPPEPQDLSS 410 SICKEEKMPR 420 AGSLESDGCP 430 KEPAKTQPMV 440 AAAATTTTTT 450 TTTVAEKYKH 460 RGEGERKDIV 470 SSSMPRPNRE 480 EPVDSRTPVT ERVS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005634 nucleus
Molecular Function GO:0010385 double-stranded methylated DNA binding
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.