Overview
| Uniprot ID | A0A0G2JZG7 |
|---|
| Protein Name | Serine--tRNA ligase, cytoplasmic |
|---|
| Gene Name | Sars1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 520 |
EGSKKKAKEVTLENQ |
Function
Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA
Protein Sequence
10
MVLDLDLFRV
20
DKGGDPALIR
30
ETQEKRFKDP
40
GLVDQLVKAD
50
SEWRRCRFRA
60
DNLNKLKNLC
70
SKTIGEKMKK
80
KEPVGEDESI
90
PEDVLNFDDL
100
TADTLAALKV
110
SQIKKVRLLV
120
DEAIQKCDGE
130
RVKLEAERFE
140
NLREIGNLLH
150
PSVPISNDED
160
ADNKVERIWG
170
DCTVRKKYSH
180
VDLVVMVDGF
190
EGEKGAVVAG
200
SRGYFLKGVL
210
VFLEQALIQY
220
ALRTLGSRGY
230
TPIYTPFFMR
240
KEVMQEVAQL
250
SQFDEELYKV
260
IGKGSEKSDD
270
SSYDEKYLIA
280
TSEQPIAALH
290
RDEWLRPEDL
300
PIKYAGFSTC
310
FRQEVGSHGR
320
DTRGIFRVHQ
330
FEKIEQFVYS
340
SPHDNKSWEM
350
FDEMITTAEE
360
FYQSLGIPYH
370
IVNIVSGSLN
380
HAASKKLDLE
390
AWFPGSGAFR
400
ELVSCSNCTD
410
YQARRLRIRY
420
GQTKKMMDKR
430
KNNLRFSTQN
440
KLEAVSSLFS
450
PKKVEFVHML
460
NATMCATTRT
470
ICAILENYQT
480
EKGIVVPEKL
490
REFMPPGLQE
500
LIPFVKPAPI
510
DQEPSKKQKK
520
QHEGSKKKAK
530
EVTLENQLQN
MEVTEA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0019899 |
enzyme binding |
| Molecular Function |
GO:0060090 |
molecular adaptor activity |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Molecular Function |
GO:0000978 |
RNA polymerase II cis-regulatory region sequence-specific DNA binding |
| Molecular Function |
GO:0098619 |
selenocysteine-tRNA ligase activity |
| Molecular Function |
GO:0004828 |
serine-tRNA ligase activity |
| Biological Process |
GO:0002181 |
cytoplasmic translation |
| Biological Process |
GO:0016525 |
negative regulation of angiogenesis |
| Biological Process |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
| Biological Process |
GO:1904046 |
negative regulation of vascular endothelial growth factor production |
| Biological Process |
GO:0001514 |
selenocysteine incorporation |
| Biological Process |
GO:0006434 |
seryl-tRNA aminoacylation |
| Biological Process |
GO:0106217 |
tRNA C3-cytosine methylation |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.