Overview
| Uniprot ID | A0A0G2K0U8 |
|---|
| Protein Name | tRNA pseudouridine synthase Pus10 |
|---|
| Gene Name | Pus10 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 69 |
EGPDPPLKKIRLHED |
Function
Protein with different functions depending on its subcellular location: involved in miRNA processing in the nucleus and acts as a tRNA pseudouridylate synthase in the cytoplasm. In the cytoplasm, acts as a pseudouridylate synthase by catalyzing synthesis of pseudouridine(54) and pseudouridine(55) from uracil-54 and uracil-55, respectively, in the psi GC loop of a subset of tRNAs. tRNA pseudouridylate synthase activity is enhanced by the presence of 1-methyladenosine at position 53-61 of tRNAs. Does not show tRNA pseudouridylate synthase activity in the nucleus. In the nucleus, promotes primary microRNAs (pri-miRNAs) processing independently of its RNA pseudouridylate synthase activity. Binds pri-miRNAs. Modulator of TRAIL/TNFSF10-induced cell death via activation of procaspase-8 and BID cleavage. Required for the progression of the apoptotic signal through intrinsic mitochondrial cell death
Protein Sequence
10
MFPLKEENRR
20
VAQLLLRSGT
30
CPRCIFRFCG
40
VDLPAPYKRP
50
YKELLDDLQT
60
FLESENRELI
70
LEGPDPPLKK
80
IRLHEDGVDT
90
LSANGEEGVS
100
VTEDENMAAT
110
KPSNWRVCNV
120
CLGILQGFCE
130
KDFITEVCQK
140
VEASGFEFTS
150
VVLSVSFPPQ
160
LSVREHAAWL
170
LVKQEMGKQS
180
LSLGRNDVVQ
190
LKEAYKWITH
200
PLFSEELGVP
210
TDGKSLFEVS
220
VVFAHPETLQ
230
DCHFLGEVCP
240
DCFKPAKNKQ
250
SVFTRMAVLK
260
ALSRIKEEDF
270
LTQFPCPPDS
280
PKTICTVLEV
290
ECTHGAVFVA
300
GRYNKYSRNL
310
PQTPWIIDGE
320
RKMESSVEEL
330
ISDHLLAVFK
340
AESFNFSSSG
350
REDVDVRTLG
360
NGRPFAVELL
370
NPHRVHFTSQ
380
EIKELQQKIN
390
KSSDKIQVRD
400
LQLVTREAIG
410
HMKEGEEEKT
420
KTYSALIWTN
430
RAIQKKDIGF
440
LDDLKDLKID
450
QKTPLRVLHR
460
RPLAVRTRAI
470
HSMKTHYLDE
480
HHFRLHLKTQ
490
AGTYIKEFVH
500
GDFGRTKPNI
510
GSLMNVTADI
520
VELDVESVDV
DWPPALDD
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0070878 |
primary miRNA binding |
| Molecular Function |
GO:0009982 |
pseudouridine synthase activity |
| Molecular Function |
GO:0106029 |
tRNA pseudouridine synthase activity |
| Molecular Function |
GO:0160148 |
tRNA pseudouridine(55) synthase activity |
| Biological Process |
GO:0031053 |
primary miRNA processing |
| Biological Process |
GO:0031119 |
tRNA pseudouridine synthesis |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.