Overview
| Uniprot ID | A0A0G2K5P9 |
| Protein Name | Propionyl-CoA carboxylase alpha chain, mitochondrial |
| Gene Name | Pcca |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 191 |
KLLAKRAKVNTIPGF |
Function
This is one of the 2 subunits of the biotin-dependent propionyl-CoA carboxylase (PCC), a mitochondrial enzyme involved in the catabolism of odd chain fatty acids, branched-chain amino acids isoleucine, threonine, methionine, and valine and other metabolites. Propionyl-CoA carboxylase catalyzes the carboxylation of propionyl-CoA/propanoyl-CoA to D-methylmalonyl-CoA/(S)-methylmalonyl-CoA. Within the holoenzyme, the alpha subunit catalyzes the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain, while the beta subunit then transfers the carboxyl group from carboxylated biotin to propionyl-CoA. Propionyl-CoA carboxylase also significantly acts on butyryl-CoA/butanoyl-CoA, which is converted to ethylmalonyl-CoA/(2S)-ethylmalonyl-CoA. Other alternative minor substrates include (2E)-butenoyl-CoA/crotonoyl-CoA
Protein Sequence
10
MAGLWVRTVA
20
LLAARRHWRR
30
SSQQLLWTLK
40
VSKAGLAWRA
50
PVYSQQCLVV
60
SRSLSSVEYE
70
PKEKTFDKIL
80
IANRGEIACR
90
VIKTCRKMGI
100
RTVAIHSDVD
110
ASSVHVKMAD
120
EAVCVGPAPT
130
SKSYLNMDAI
140
MEAIKKTGAQ
150
AVHPGYGFLS
160
ENKEFAKCLA
170
AEDVTFIGPD
180
THAIQAMGDK
190
IESKLLAKRA
200
KVNTIPGFDG
210
VVKDADEAVR
220
IAREIGYPVM
230
IKASAGGGGK
240
GMRIAWDDEE
250
TRDGFRFSSQ
260
EAASSFGDDR
270
LLIEKFIDNP
280
RHIEIQVLGD
290
KHGNALWLNE
300
RECSIQRRNQ
310
KVVEEAPSIF
320
LDPETRRAMG
330
EQAVALAKAV
340
KYSSAGTVEF
350
LVDSQKNFYF
360
LEMNTRLQVE
370
HPVTECITGL
380
DLVQEMILVA
390
KGYPLRHKQE
400
DIPISGWAVE
410
CRVYAEDPYK
420
SFGLPSIGRL
430
SQYQEPIHLP
440
GVTLDLCHTF
450
MLENLVTYGS
460
DRAEALKRME
470
DALDSYVIRG
480
VTHNIPLLRE
490
VIINTRFVKG
500
DISTKFLSDV
510
YPDGFKGHML
520
TPSERDQLLA
530
IASSLFVASQ
540
LRAQRFQEHS
550
RVPVIRPDVA
560
KWELSVKLHD
570
EDHTVVASNN
580
GPTFNVEVDG
590
SKLNVTSTWN
600
LASPLLSVNV
610
DGTQRTVQCL
620
SRDAGGNMSI
630
QFLGTVYKVH
640
ILTKLAAELN
650
KFMLEKVPKD
660
TSSTLCSPMP
670
GVVVAVSVKP
680
GDMVAEGQEI
690
CVIEAMKMQN
700
SMTAGKMGKV
710
KLVHCKAGDT
720
VGEGDLLVEL
E
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0004658 |
propionyl-CoA carboxylase activity |
| Biological Process |
GO:0019752 |
carboxylic acid metabolic process |
| Biological Process |
GO:0016042 |
lipid catabolic process |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.