Overview
| Uniprot ID | A0A0H2UHC3 |
|---|
| Protein Name | Tyrosyl-DNA phosphodiesterase 1 |
|---|
| Gene Name | Tdp1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 131 |
GVPPQEKKYAPSANV |
Function
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate
Protein Sequence
10
MTKIGIVCAR
20
MAHRSTMSQE
30
SSYGKWTISS
40
SDDSEDEKPK
50
PVKPSTSSGP
60
QAGQGVSKEP
70
TYTCSEARKA
80
AHKRQISPVK
90
FNNTDSVLPH
100
KKQKMDSPEG
110
LGWCLSSSDD
120
EQQPDVTQQE
130
QPKGVPPQEK
140
KYAPSANVTT
150
AQKVEDRSPP
160
DSHRAQRADE
170
EYETSGEGQD
180
IWDMLDKENP
190
FQFYLTRVSG
200
IKAKYNSKAL
210
HIKDILSPLF
220
GTLVSSAQFN
230
YCFDVNWLIK
240
QYPPEFRKKP
250
ILLVHGDKRE
260
AKADLHAQAK
270
PYANISLCQA
280
KLDIAFGTHH
290
TKMMLLLYEE
300
GLRVVIHTSN
310
LIREDWHQKT
320
QGIWLSPLYP
330
RIYQGNHTSG
340
ESSTHFKADL
350
TSYLMAYNAP
360
PLQEWIDIIQ
370
EHDLSETNVY
380
LIGSTPGRFQ
390
GSHKDNWGHF
400
RLRKLLQAHA
410
PSAPRGECWP
420
VVGQFSSIGS
430
LGPDESKWLC
440
SEFKESLLAV
450
REEGRTPGRS
460
AVPLHLIYPS
470
VENVRTSLEG
480
YPAGGSLPYG
490
IQTAEKQRWL
500
HPYFHKWSAE
510
TSGRSNAMPH
520
IKTYMRPSPD
530
FSKLAWFLVT
540
SANLSKAAWG
550
ALEKNGAQLM
560
IRSYELGVLF
570
LPSAFGLDTF
580
KVKQKFFSSS
590
SEPMASFPVP
600
YDLPPELYGS
610
KDRPWIWNIP
620
YVKAPDTHGN
MWVPS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0004527 |
exonuclease activity |
| Molecular Function |
GO:0008081 |
phosphoric diester hydrolase activity |
| Biological Process |
GO:0000012 |
single strand break repair |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.