Search Results
Overview
| Uniprot ID | A0A0H2UHG0 |
|---|---|
| Protein Name | Tyrosine--tRNA ligase, cytoplasmic |
| Gene Name | Yars1 |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 155 | LEKLKFTKGTDYQLS |
| 182 | LVTQHDAKKAGAEVV |
Function
Tyrosine--tRNA ligase that catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). Also acts as a positive regulator of poly-ADP-ribosylation in the nucleus, independently of its tyrosine--tRNA ligase activity. Activity is switched upon resveratrol-binding: resveratrol strongly inhibits the tyrosine--tRNA ligase activity and promotes relocalization to the nucleus, where YARS1 specifically stimulates the poly-ADP-ribosyltransferase activity of PARP1
Protein Sequence
10
MQPDGTSVTV
20
PGTRRRRRTR
30
KGRGCRLSAG
40
NRESGAMGDA
50
PSPEEKLHLI
60
TRNLQEVLGE
70
EKLKEILKER
80
ELKVYWGTAT
90
TGKPHVAYFV
100
PMSKIADFLK
110
AGCEVTILFA
120
DLHAYLDNMK
130
APWELLELRT
140
SYYENVIKAM
150
LESIGVPLEK
160
LKFTKGTDYQ
170
LSKEYTLDVY
180
RLSSLVTQHD
190
AKKAGAEVVK
200
QVEHPLLSGL
210
LYPGLQYLPT
220
LGYSKRVHLM
230
NPMVPGLTGS
240
KMSSSEEESK
250
IDLLDRKEDV
260
KKKLKKAFCE
270
PGNVENNGVL
280
SFVKHVLFPL
290
KSEFVILRDE
300
KWGGNKTYTI
310
YQELEKDFAA
320
EVVHPGDLKN
330
SVEVALNKLL
340
DPIREKFNTP
350
ALKKLASAAY
360
PDPSKQKPTA
370
KGPAKSSEPE
380
EIIPSRLDIR
390
VGKILSVEKH
400
PDADSLYVEK
410
IDVGEAEPRT
420
VVSGLVQFVP
430
KEELQDRLVV
440
VLCNLKPQKM
450
RGVDSQGMLL
460
CASVEGVSRQ
470
VEPLDPPAGS
480
APGERVFVQG
490
YEKGQPDEEL
500
KPKKKVFEKL
510
QADFKISDDC
520
VAQWKQTNFM
530
TKLGFVSCKS
LKGGNIS
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Molecular Function | GO:0004812 | aminoacyl-tRNA ligase activity |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0000049 | tRNA binding |
| Biological Process | GO:0006418 | tRNA aminoacylation for protein translation |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.