Overview
| Uniprot ID | A0A0H2UHJ4 |
|---|
| Protein Name | Integrator complex subunit 12 |
|---|
| Gene Name | Ints12 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 252 |
TVKDPLVKKPETKLK |
| 314 |
SAGPSTAKLNSTAQN |
| 346 |
TGLATSSKGGIGSKI |
| 69 |
PPKISSTKSLSIKQE |
Function
Component of the integrator complex, a multiprotein complex that terminates RNA polymerase II (Pol II) transcription in the promoter-proximal region of genes. The integrator complex provides a quality checkpoint during transcription elongation by driving premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: the complex terminates transcription by (1) catalyzing dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, (2) degrading the exiting nascent RNA transcript via endonuclease activity and (3) promoting the release of Pol II from bound DNA. The integrator complex is also involved in terminating the synthesis of non-coding Pol II transcripts, such as enhancer RNAs (eRNAs), small nuclear RNAs (snRNAs), telomerase RNAs and long non-coding RNAs (lncRNAs). Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the integrator complex
Protein Sequence
10
MQLVLAMAAT
20
VNLELDPIFL
30
KALGFLHSKS
40
KDSAEKLKTL
50
LDESLARGID
60
ASYRPAQKDV
70
EPPKISSTKS
80
LSIKQEPKTS
90
SSLPSGSSNG
100
KVLAAEKLKK
110
EAEKRPADKM
120
KDATEGVDVP
130
KKPRLEKPET
140
RSSPITVQTS
150
KDLAMADLSS
160
FEETSADDFA
170
MEMGLACVVC
180
RQMTVASGNQ
190
LVECQECHNL
200
YHQDCHKPQV
210
TDKEVNDPRL
220
VWYCARCTRQ
230
MKRMAQKTQK
240
PPQKPAPTVV
250
SVAPTVKDPL
260
VKKPETKLKQ
270
ETTFLAFKRT
280
EVKPSTVISG
290
NSSSNNVSSS
300
VTSGLTGWAA
310
FAAKTSSAGP
320
STAKLNSTAQ
330
NSSGKPAASS
340
ASQKPVGLTG
350
LATSSKGGIG
360
SKIGSSNSTS
370
PSVPLKPLPP
380
LTLGKTGLSR
390
SVSCDNVSKV
400
GLPSPSSLVP
410
GGSSQLSGNG
420
NSATAGPTGS
430
ITSKTTSETS
440
SSTSASLKGP
450
TSQESQLNAM
460
KRLQMVKKKA
AQKKLKK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0160232 |
INTAC complex |
| Cellular Component |
GO:0032039 |
integrator complex |
| Cellular Component |
GO:0016604 |
nuclear body |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0034243 |
regulation of transcription elongation by RNA polymerase II |
| Biological Process |
GO:0160240 |
RNA polymerase II transcription initiation surveillance |
| Biological Process |
GO:0016180 |
snRNA processing |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.