Overview
| Uniprot ID | A0A0H2UHV4 |
| Protein Name | Eukaryotic translation initiation factor 5B |
| Gene Name | Eif5b |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 330 |
DDKEKEKKKGPSKST |
| 39 |
EQEPQKGKGKKKKEK |
Function
Plays a role in translation initiation. Ribosome-dependent GTPase that promotes the joining of the 60S ribosomal subunit to the pre-initiation complex to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. Together with eIF1A (EIF1AX), actively orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after formation of the 80S initiation complex. Its GTPase activity is not essential for ribosomal subunits joining, but GTP hydrolysis is needed for eIF1A (EIF1AX) ejection quickly followed by EIF5B release to form elongation-competent ribosomes. In contrast to its procaryotic homolog, does not promote recruitment of Met-rRNA to the small ribosomal subunit
Protein Sequence
10
YGRRQSLVSS
20
TKDDTDLGAL
30
AAEIEGAGAA
40
KEQEPQKGKG
50
KKKKEKKKQD
60
FDENDILREL
70
EELSLEAQGI
80
GADRDAATVK
90
PTENNEEESA
100
SKQDKKKKGQ
110
KGKKTSFDEN
120
DSEELEDKDS
130
KSKKPARPNS
140
EVLLSGSEDA
150
DDPNKLSKKG
160
KKAQKSTKKR
170
DGSEEDEDNS
180
KRSKERSRVN
190
SSGESGGESD
200
EFLQSRKGQK
210
KNQKNKSVPT
220
IDSGNEDDDS
230
SFKIKTVAQK
240
KAEKKERERK
250
KREEEKAKLR
260
KVKEKEELEK
270
GRKEQSKQRE
280
PQKRPDEEVL
290
VLRGTPDAGA
300
ASEEKGDIAA
310
TLEDDNEGDK
320
KKKDKKKKKT
330
EKDDKEKEKK
340
KGPSKSTVKA
350
IQEALAKLRE
360
EEERQKREEE
370
ERIKRLEELE
380
AKRKEEERLE
390
QEKRERKKQK
400
EKERKERLKK
410
EGKLLTKSQR
420
EARARAEVTL
430
RHLQAQGVEV
440
PSKDSLPKKR
450
PVYEDKKKKK
460
TPQQLESKEA
470
LETVEVSAPV
480
EVVDQGVPEK
490
EETPPSVDAE
500
EDEETEDAGL
510
DDWEAMASDE
520
EREKEGNMIH
530
IEVEENPEEE
540
EEEEEDEDEE
550
DSEDEEDEGD
560
SEGSDGDEED
570
YKLSDEKDLG
580
KAGDTKPNKD
590
ASSDSEYDSD
600
DDRTKEERAY
610
DKAKRRIEKR
620
RLEHGKNVNT
630
EKLRAPIICV
640
LGHVDTGKTK
650
ILDKLRHTHV
660
QDGEAGGITQ
670
QIGATNVPLE
680
AINEQTKMIK
690
NFDRENVRIP
700
GMLIIDTPGH
710
ESFSNLRNRG
720
SSLCDIAILV
730
VDIMHGLEPQ
740
TIESINILKS
750
KKCPFIVALN
760
KIDRLYDWKK
770
SPDSDVAVTL
780
KKQKKNTKDE
790
FEERAKAIIV
800
EFAQQGLNAA
810
LFYENKDPRT
820
FVSLVPTSAH
830
TGDGMGSLIY
840
LLVELTQTML
850
SKRLAHCEEL
860
RAQVMEVKAL
870
PGMGTTIDVI
880
LINGRLKEGD
890
TIIVPGVEGP
900
IVTQIRGLLL
910
PPPMKELRVK
920
NQYEKHKEVE
930
AAQGVKILGK
940
DLEKTLAGLP
950
LLVAYKDDEI
960
PVLKDELIHE
970
LKQTLNAIKL
980
EEKGVYVQAS
990
TLGSLEALLE
1000
FLKTSEVPYA
1010
GINIGPVHKK
1020
DVMKASVMLE
1030
HDPQYAVILA
1040
FDVRIERDAQ
1050
EMADSLGVRI
1060
FSAEIIYHLF
1070
DAFTKYRQDY
1080
KKQKQEEFKH
1090
IAVFPCKMKI
1100
LPQYIFNSRD
1110
PIVIGVTVEA
1120
GQVKQGTPMC
1130
VPSKNFVDIG
1140
IVTSIEINHK
1150
QVDVAKKGQE
1160
VCVKIEPIPG
1170
ESPKMFGRHF
1180
EATDILVSKI
1190
SRQSIDALKD
1200
WFRDEMQKSD
1210
WQLIVELKKV
FEII
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Molecular Function |
GO:0005525 |
GTP binding |
| Molecular Function |
GO:0003924 |
GTPase activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.