Overview
| Uniprot ID | A0A0H2UHZ6 |
|---|
| Protein Name | Poly(U)-binding-splicing factor PUF60 |
|---|
| Gene Name | Puf60 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 437 |
ARHMVMQKLLRKQES |
Function
DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with RO60. Binds to poly(U) RNA
Protein Sequence
10
MMPLGNLEFA
20
AEQLPCFLLQ
30
GTESIKMENG
40
QSTGTKLGLP
50
PLTPEQQEAL
60
QKAKKYAMEQ
70
SIKSVLVKQT
80
IAHQQQQLTN
90
LQMAAVTMGF
100
GDPLSPLQSM
110
AAQRQRALAI
120
MCRVYVGSIY
130
YELGEDTIRQ
140
AFAPFGPIKS
150
IDMSWDSVTM
160
KHKGFAFVEY
170
EVPEAAQLAL
180
EQMNSVMLGG
190
RNIKVGRPSN
200
IGQAQPIIDQ
210
LAEEARAFNR
220
IYVASVHQDL
230
SDDDIKSVFE
240
AFGKIKSCTL
250
ARDPTTGKHK
260
GYGFIEYEKA
270
QSSQDAVSSM
280
NLFDLGGQYL
290
RVGKAVTPPM
300
PLLTPATPGG
310
LPPAAAVAAA
320
AATAKITAQE
330
AVAGAAVLGT
340
LATPGLVSPA
350
LTLAQPLGAL
360
PQAVMAAQAP
370
GVITGVTPAR
380
PPIPVTIPSV
390
GVVNPILASP
400
PTLGLLEPKK
410
EKEEEELFPE
420
SERPEMLSEQ
430
EHMSISGSSA
440
RHMVMQKLLR
450
KQESTVMVLR
460
NMVDPKDIDD
470
DLEGEVTEEC
480
GKFGAVNRVI
490
IYQEKQGEEE
500
DAEIIVKIFV
510
EFSMASETHK
520
AIQALNGRWF
530
GGRKVVAEVY
540
DQERFDNSDL
SA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003723 |
RNA binding |
| Biological Process |
GO:0006397 |
mRNA processing |
| Biological Process |
GO:0000381 |
regulation of alternative mRNA splicing, via spliceosome |
| Biological Process |
GO:0008380 |
RNA splicing |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.