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Overview

Uniprot IDA0A0S2Z4R1
Protein NameTyrosine--tRNA ligase
Gene NameYARS
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
412 GLVQFVPKEELQDRL

Function

Tyrosine--tRNA ligase that catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr). Also acts as a positive regulator of poly-ADP-ribosylation in the nucleus, independently of its tyrosine--tRNA ligase activity. Activity is switched upon resveratrol-binding: resveratrol strongly inhibits the tyrosine--tRNA ligase activity and promotes relocalization to the nucleus, where YARS1 specifically stimulates the poly-ADP-ribosyltransferase activity of PARP1

Protein Sequence

10 MGDAPSPEEK 20 LHLITRNLQE 30 VLGEEKLKEI 40 LKERELKIYW 50 GTATTGKPHV 60 AYFVPMSKIA 70 DFLKAGCEVT 80 ILFADLHAYL 90 DNMKAPWELL 100 ELRVSYYENV 110 IKAMLESIGV 120 PLEKLKFIKG 130 TDYQLSKEYT 140 LDVYRLSSVV 150 TQHDSKKAGA 160 EVVKQVEHPL 170 LSGLLYPGLQ 180 ALDEEYLKVD 190 AQFGGIDQRK 200 IFTFAEKYLP 210 ALGYSKRVHL 220 MNPMVPGLTG 230 SKMSSSEEES 240 KIDLLDRKED 250 VKKKLKKAFC 260 EPGNVENNGV 270 LSFIKHVLFP 280 LKSEFVILRD 290 EKWGGNKTYT 300 AYVDLEKDFA 310 AEVVHPGDLK 320 NSVEVALNKL 330 LDPIREKFNT 340 PALKKLASAA 350 YPDPSKQKPM 360 AKGPAKNSEP 370 EEVIPSRLDI 380 RVGKIITVEK 390 HPDADSLYVE 400 KIDVGEAEPR 410 TVVSGLVQFV 420 PKEELQDRLV 430 VVLCNLKPQK 440 MRGVESQGML 450 LCASIEGINR 460 QVEPLDPPAG 470 SAPGEHVFVK 480 GYEKGQPDEE 490 LKPKKKVFEK 500 LQADFKISEE 510 CIAQWKQTNF 520 MTKLGSISCK SLKGGNIS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0016604 nuclear body
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0000049 tRNA binding
Molecular Function GO:0004831 tyrosine-tRNA ligase activity
Biological Process GO:0006950 response to stress
Biological Process GO:0006437 tyrosyl-tRNA aminoacylation

Reference

PMID: N/A