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Overview

Uniprot IDA0A286X7L6
Protein NameMacrophage migration inhibitory factor
Gene NameMIF
OrganismCavia porcellus

Kla Sites from experimental identification

Position Flanking peptide
78 AQNRSYSKLLCGLLS

Function

Pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity

Protein Sequence

10 MPMFILNTNV 20 PRSSVPDGFL 30 SELTQQLAQA 40 TGKPAQYIAV 50 HVVPDQLMTF 60 AGSSEPCALC 70 SLHSIGKIGG 80 AQNRSYSKLL 90 CGLLSERLRI 100 SPDRVYINYY 110 DMNAANVGWN STTFG

Gene Ontology

Classification GO ID Description
Cellular Component GO:0009986 cell surface
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0042056 chemoattractant activity
Molecular Function GO:0005125 cytokine activity
Molecular Function GO:0005126 cytokine receptor binding
Molecular Function GO:0004167 dopachrome isomerase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0050178 phenylpyruvate tautomerase activity
Molecular Function GO:0002020 protease binding
Biological Process GO:0007166 cell surface receptor signaling pathway
Biological Process GO:0006954 inflammatory response
Biological Process GO:0045087 innate immune response
Biological Process GO:2000773 negative regulation of cellular senescence
Biological Process GO:0043518 negative regulation of DNA damage response, signal transduction by p53 class mediator
Biological Process GO:0010629 negative regulation of gene expression
Biological Process GO:1902166 negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
Biological Process GO:0010760 negative regulation of macrophage chemotaxis
Biological Process GO:0002906 negative regulation of mature B cell apoptotic process
Biological Process GO:0033033 negative regulation of myeloid cell apoptotic process
Biological Process GO:0051248 negative regulation of protein metabolic process
Biological Process GO:0090238 positive regulation of arachidonate secretion
Biological Process GO:0030890 positive regulation of B cell proliferation
Biological Process GO:0141163 positive regulation of cAMP/PKA signal transduction
Biological Process GO:2000343 positive regulation of chemokine (C-X-C motif) ligand 2 production
Biological Process GO:0070374 positive regulation of ERK1 and ERK2 cascade
Biological Process GO:0048146 positive regulation of fibroblast proliferation
Biological Process GO:0031666 positive regulation of lipopolysaccharide-mediated signaling pathway
Biological Process GO:0061081 positive regulation of myeloid leukocyte cytokine production involved in immune response
Biological Process GO:0042327 positive regulation of phosphorylation
Biological Process GO:0061078 positive regulation of prostaglandin secretion involved in immune response
Biological Process GO:0032760 positive regulation of tumor necrosis factor production
Biological Process GO:0001516 prostaglandin biosynthetic process
Biological Process GO:0070207 protein homotrimerization

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.