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Overview

Uniprot IDA0A286XFH5
Protein NameDNA repair nuclease/redox regulator APEX1
Gene NameAPEX1
OrganismCavia porcellus

Kla Sites from experimental identification

Position Flanking peptide
24 PKREPEAKKSKAGPK
32 KSKAGPKKNEKEEAG
7 *MPKRGKKGSVAEDG

Function

Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends

Protein Sequence

10 MPKRGKKGSV 20 AEDGEEPKRE 30 PEAKKSKAGP 40 KKNEKEEAGE 50 GPALYEDPPD 60 KKTSPSGKSA 70 TLKICSWNVD 80 GLRAWIKKKG 90 LDWVKEEAPD 100 ILCLQETKCS 110 ENKLPAELQE 120 LSELSHQYWS 130 APSDKEGYSG 140 VGLLSRQCPI 150 KVSYGIGEEE 160 HDQEGRVIVA 170 EFDSFVLVTA 180 YVPNAGRGLV 190 RLEYRQRWDE 200 AFRKFLKGLA 210 SRKPLVLCGD 220 LNVAHEEIDL 230 RNPKGNKKNA 240 GFTPQERQGF 250 GELLQAVPLA 260 DSFRHLYPNA 270 AYAYTFWTYM 280 MNARSKNVGW 290 RLDYFLLSHS 300 LLPALCDSKI 310 RSKALGSDHC PITLYLAL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0048471 perinuclear region of cytoplasm
Molecular Function GO:0031490 chromatin DNA binding
Molecular Function GO:0052720 class II DNA-(apurinic or apyrimidinic site) endonuclease activity
Molecular Function GO:0033892 deoxyribonuclease (pyrimidine dimer) activity
Molecular Function GO:0140431 DNA-(abasic site) binding
Molecular Function GO:0008311 double-stranded DNA 3'-5' DNA exonuclease activity
Molecular Function GO:0003691 double-stranded telomeric DNA binding
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0016491 oxidoreductase activity
Molecular Function GO:0090580 phosphodiesterase activity, acting on 3'-phosphoglycolate-terminated DNA strands
Molecular Function GO:0003713 transcription coactivator activity
Biological Process GO:0006308 DNA catabolic process
Biological Process GO:0006310 DNA recombination
Biological Process GO:0044029 positive regulation of gene expression via chromosomal CpG island demethylation
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0042981 regulation of apoptotic process
Biological Process GO:0043488 regulation of mRNA stability
Biological Process GO:0097698 telomere maintenance via base-excision repair

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.