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Overview

Uniprot IDA0A286XM88
Protein NametRNA-splicing ligase complex subunit RTRAF
Gene NameRTRAF
OrganismCavia porcellus

Kla Sites from experimental identification

Position Flanking peptide
108 DLTPDNAKNIDNATK

Function

Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Could also play a role in RNA transport

Protein Sequence

10 MFRRKLTALD 20 YHNPAGFNCK 30 DETEFRNFIV 40 WLEDQKIRHY 50 KIEDRGNLRN 60 IPSSDWPKFF 70 EKYLRDVDCP 80 FKIQDRQEAI 90 DWLLGLAVRL 100 EYGDNAEKYK 110 DLTPDNAKNI 120 DNATKNAEPL 130 INLDVNNPDF 140 KAGVMALANL 150 LQIQRHDDYL 160 VMLKAIRILV 170 QERLTQDAVA 180 KANQTKEGLP 190 VALDKHILGF 200 DTGDAVLNEA 210 AQILRLLHIE 220 ELRELQTKIN 230 EAIVAVQAII 240 ADPKTDHRLG KVGR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005813 centrosome
Cellular Component GO:0072686 mitotic spindle
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0048471 perinuclear region of cytoplasm
Cellular Component GO:0072669 tRNA-splicing ligase complex
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0000993 RNA polymerase II complex binding
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0006388 tRNA splicing, via endonucleolytic cleavage and ligation

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.