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Overview

Uniprot IDA0A286XVD2
Protein NameNEDD8-conjugating enzyme UBC12
Gene NameUBE2M
OrganismCavia porcellus

Kla Sites from experimental identification

Position Flanking peptide
8 MIKLFSLKQQKKEEE

Function

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation

Protein Sequence

10 MIKLFSLKQQ 20 KKEEESAGGT 30 KGSSKKASAA 40 QLRIQKDINE 50 LNLPKTCDIS 60 FSDPRRPPNF 70 KLVICPMEGF 80 YKSGKFVFSF 90 KVGQGYPHAP 100 PKVKCETMVY 110 HPNIALEGNV 120 CLNILREDWK 130 PVLTINSIIY 140 GLQYLFLEPN 150 PEDPLNKEAA 160 EVLQNNRRLF 170 EQNVQRSMRG 180 GYIGSIYFER CLK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0098978 glutamatergic synapse
Cellular Component GO:0016604 nuclear body
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0098794 postsynapse
Cellular Component GO:0098793 presynapse
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0061654 NEDD8 conjugating enzyme activity
Molecular Function GO:0004842 ubiquitin-protein transferase activity
Biological Process GO:0045116 protein neddylation
Biological Process GO:0150052 regulation of postsynapse assembly

Reference

[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.