Overview
| Uniprot ID | A0A286XXY9 |
|---|
| Protein Name | Splicing factor 3A subunit 2 |
|---|
| Gene Name | SF3A2 |
|---|
| Organism | Cavia porcellus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 10 |
FQHRPGGKTGSGGVA |
| 76 |
YLAHTQGKKHQTNLA |
Function
Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A2 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes, including the Bact complex. Interacts directly with the duplex formed by U2 snRNA and the intron
Protein Sequence
10
MDFQHRPGGK
20
TGSGGVASSS
30
ESNRDRRERL
40
RQLALETIDI
50
NKDPYFMKNH
60
LGSYECKLCL
70
TLHNNEGSYL
80
AHTQGKKHQT
90
NLARRAAKEA
100
KEAPAQPAPE
110
KVKVEVKKFV
120
KIGRPGYKVT
130
KQRDTEMGQQ
140
SLLFQIDYPE
150
IAEGIMPRHR
160
FMSAYEQRIE
170
PPDRRWQYLL
180
MAAEPYETIA
190
FKVPSREIDK
200
AEGKFWTHWN
210
RETKQFFLQF
220
HFKMEKPPAP
230
PSLPAGPPGV
240
KRPPPPLMNG
250
LPPRPPLPES
260
LPPPPPGGLP
270
LPPMPPTGPA
280
PSGPPGPPQL
290
PPPAPGVHPP
300
TPVVHPPASG
310
VHPPAPGVHP
320
PAPVVHAPTS
330
AVHPAAPGVH
340
PPAPGVHPPA
350
PGVHPPAPGV
360
HPPPAAGVHP
370
QTPGVHPPAP
380
AVHPQAPGVH
390
PAAPGIHPQA
400
PGVHPQPPGV
410
HPAAPGVHPQ
420
PPGVHPANPG
430
VHPPTPMPPM
440
LRPPLPSEGP
450
GNIPPPPPTN
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0071013 |
catalytic step 2 spliceosome |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005686 |
U2 snRNP |
| Cellular Component |
GO:0071005 |
U2-type precatalytic spliceosome |
| Cellular Component |
GO:0071004 |
U2-type prespliceosome |
| Molecular Function |
GO:0003676 |
nucleic acid binding |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:1903241 |
U2-type prespliceosome assembly |
Reference
[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.