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Overview

Uniprot IDA0A286ZK63
Protein NameEukaryotic translation initiation factor 5A
Gene NameEIF5A
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
109 NMDVPNIKRNDFQLI
145 LPEGDLGKEIEQKYD
51 MQCSALRKNGFVVLK
58 KNGFVVLKGRPCKIV
63 VLKGRPCKIVEMSTS
91 GIDIFTGKKYEDICP
92 IDIFTGKKYEDICPS

Function

Translation factor that promotes translation elongation and termination, particularly upon ribosome stalling at specific amino acid sequence contexts. Binds between the exit (E) and peptidyl (P) site of the ribosome and promotes rescue of stalled ribosome: specifically required for efficient translation of polyproline-containing peptides as well as other motifs that stall the ribosome. Acts as ribosome quality control (RQC) cofactor by joining the RQC complex to facilitate peptidyl transfer during CAT tailing step

Protein Sequence

10 MGSETRGPPH 20 STSFLKRLES 30 KPLKMADDLD 40 FETGDAGASA 50 TFPMQCSALR 60 KNGFVVLKGR 70 PCKIVEMSTS 80 KTGKHGHAKV 90 HLVGIDIFTG 100 KKYEDICPST 110 HNMDVPNIKR 120 NDFQLIGIQD 130 GYLSLLQDSG 140 EVREDLRLPE 150 GDLGKEIEQK 160 YDCGEEILIT 170 VLSAMTEEAA VAIKAMAK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005789 endoplasmic reticulum membrane
Molecular Function GO:0043022 ribosome binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003746 translation elongation factor activity
Biological Process GO:0045901 positive regulation of translational elongation
Biological Process GO:0045905 positive regulation of translational termination

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.