Overview
| Uniprot ID | A0A286ZKD4 |
| Protein Name | Electrogenic aspartate/glutamate antiporter SLC25A13, mitochondrial |
| Gene Name | SLC25A13 |
| Organism | Sus scrofa |
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 23 |
ELRTIFLKYASIEKN |
| 235 |
NNMELIRKIYSTLAG |
| 379 |
KNSFDCFKKVLRYEG |
| 408 |
VAPEKAIKLTVNDFV |
| 487 |
FGIYKGAKACFLRDI |
| 5 |
***MAVAKVALTKRA |
Function
Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle. Substrate exchange across the membrane occurs consecutively with one substrate being transported first, then dissociating from the substrate binding site before the second substrate binds for transport in the opposite direction. Also mediates the uptake of L-cysteinesulfinate (3-sulfino-L-alanine) by mitochondria in exchange for L-glutamate and proton. Can also exchange L-cysteinesulfinate with aspartate in their anionic form without any proton translocation. Lacks transport activity towards gamma-aminobutyric acid (GABA)
Protein Sequence
10
MAVAKVALTK
20
RADPAELRTI
30
FLKYASIEKN
40
GEFFMSPNDF
50
VTRYLNIFGE
60
SQPNPKTVEL
70
LSGVVDQTKD
80
GLISFQEFVA
90
FESVLCAPDA
100
LFMVVFQLFD
110
KAGKGEVTFE
120
DVKQVFEQTT
130
IHQRIPFNWD
140
SEFVQLHFGK
150
ERKRHLTYAE
160
FTQFLLEIQL
170
EHAKQAFVQR
180
DSAKTGKVTA
190
IDFRDIMVTI
200
RPHVLTPFVE
210
ECLVAAAGGT
220
TSHQVSFSYF
230
NGFNSLLNNM
240
ELIRKIYSTL
250
AGNRKDVEVT
260
KEEFVLAAQK
270
FGQVTPMEVD
280
ILFQLADLYE
290
PRGRMTLADI
300
ERIAPLEEGT
310
LPFNLAEAQR
320
QKASVDSSRP
330
VLLQIAESAY
340
RFGLGSVAGA
350
VGATAVYPID
360
LVKTRMQNQR
370
STGSFVGELM
380
YKNSFDCFKK
390
VLRYEGFFGL
400
YRGLLPQLLG
410
VAPEKAIKLT
420
VNDFVRDKFM
430
RKDGSVPLAA
440
EILAGGCAGG
450
SQVIFTNPLE
460
IVKIRLQVAG
470
EITTGPRVSA
480
LSVLRDLGFF
490
GIYKGAKACF
500
LRDIPFSAIY
510
FPCYAHVKAS
520
LASEDGQISP
530
GSLLLAGAIA
540
GMPAASLVTP
550
ADVIKTRLQV
560
AARAGQTTYS
570
GVIDCFWKIL
580
REEGPKALWK
590
GAGARVFRSS
600
PQFGVTLLTY
610
ELLQRWFYID
620
FGGVKPMGSE
630
PVPKSRITLP
640
APNPDHVGGY
650
KLAVATFAGI
660
ENKFGLYLPL
670
FKPSASTSQA
680
LREAHRRTSP
GLLL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005743 |
mitochondrial inner membrane |
| Molecular Function |
GO:0000514 |
3-sulfino-L-alanine: proton, glutamate antiporter activity |
| Molecular Function |
GO:0000515 |
aspartate:glutamate, proton antiporter activity |
| Molecular Function |
GO:0005509 |
calcium ion binding |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0015183 |
L-aspartate transmembrane transporter activity |
| Molecular Function |
GO:0005313 |
L-glutamate transmembrane transporter activity |
| Biological Process |
GO:0015810 |
aspartate transmembrane transport |
| Biological Process |
GO:0006754 |
ATP biosynthetic process |
| Biological Process |
GO:0045333 |
cellular respiration |
| Biological Process |
GO:0006094 |
gluconeogenesis |
| Biological Process |
GO:0015813 |
L-glutamate transmembrane transport |
| Biological Process |
GO:0043490 |
malate-aspartate shuttle |
| Biological Process |
GO:0051592 |
response to calcium ion |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.