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Overview

Uniprot IDA0A286ZNE7
Protein NameSqualene synthase
Gene NameFDFT1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
45 SSLKTCYKYLNQTSR

Function

Catalyzes the condensation of 2 farnesyl pyrophosphate (FPP) moieties to form squalene. Proceeds in two distinct steps. In the first half-reaction, two molecules of FPP react to form the stable presqualene diphosphate intermediate (PSQPP), with concomitant release of a proton and a molecule of inorganic diphosphate. In the second half-reaction, PSQPP undergoes heterolysis, isomerization, and reduction with NADPH or NADH to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway

Protein Sequence

10 MEFVKCLGHP 20 EEFYNLLRFQ 30 MGGRRKVIPK 40 MNQDSLSSSL 50 KTCYKYLNQT 60 SRSFAAVIQA 70 LDGEMRHAVC 80 IFYLVLRALD 90 TLEDDMTISI 100 EKKIPLLHNF 110 HLHLYESDWR 120 FTESKEKDRQ 130 VLEDFPTIAL 140 EFRNLAEKYQ 150 VVIVDVCRKM 160 GFGMAEFLNK 170 HVTSEREWDK 180 YCHYVAGLVG 190 IGLSRLFSAS 200 ELEDPLVGED 210 TERANSMGLF 220 LQKTNIIRDY 230 LEDQQEGREF 240 WPQEVWSKYV 250 KKLGDFAKPE 260 NVDLAVQCLN 270 ELVTSTLHHI 280 PDVITYLSRL 290 RNQSIFNFCA 300 IPQVMAIATL 310 AACYNNQQVF 320 KGVVKIRKGQ 330 AVTLMMDATN 340 MPAVKAIIHQ 350 YMEEIYHRIP 360 DSDPYSAKTR 370 QIISTIRTQN 380 LPNCQLISRS 390 HYSPIYLSFV 400 MLLAALSWQY 410 LSTLSQVTED YVQTGEH

Gene Ontology

Classification GO ID Description
Cellular Component GO:0098554 cytoplasmic side of endoplasmic reticulum membrane
Cellular Component GO:0005789 endoplasmic reticulum membrane
Molecular Function GO:0055056 D-glucose transmembrane transporter activity
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0051996 squalene synthase [NAD(P)H] activity
Biological Process GO:0006695 cholesterol biosynthetic process
Biological Process GO:0033489 cholesterol biosynthetic process via desmosterol
Biological Process GO:0033490 cholesterol biosynthetic process via lathosterol
Biological Process GO:0045338 farnesyl diphosphate metabolic process
Biological Process GO:0036197 zymosterol biosynthetic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.