Overview
| Uniprot ID | A0A286ZU22 |
|---|
| Protein Name | AP-2 complex subunit mu |
|---|
| Gene Name | AP2M1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 224 |
NDKIVIEKQGKGTAD |
| 256 |
HQCVRLSKFDSERSI |
| 281 |
LMRYRTTKDIILPFR |
| 312 |
VVIKSNFKPSLLAQK |
| 319 |
KPSLLAQKIEVRIPT |
| 345 |
MKGKAKYKASENAIV |
| 405 |
GLKVRYLKVFEPKLN |
| 420 |
YSDHDVIKWVRYIGR |
| 59 |
RTSFFHVKRSNIWLA |
Function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules
Protein Sequence
10
MIGGLFIYNH
20
KGEVLISRVY
30
RDDIGRNAVD
40
AFRVNVIHAR
50
QQVRSPVTNI
60
ARTSFFHVKR
70
SNIWLAAVTK
80
QNVNAAMVFE
90
FLYKMCDVMA
100
AYFGKISEEN
110
IKNNFVLIYE
120
LLDEILDFGY
130
PQNSETGALK
140
TFITQQGIKS
150
QMQTKEEQSQ
160
ITSQVTGQIG
170
WRREGIKYRR
180
NELFLDVLES
190
VNLLMSPQGQ
200
VLSAHVSGRV
210
VMKSYLSGMP
220
ECKFGMNDKI
230
VIEKQGKGTA
240
DETSKSGKQS
250
IAIDDCTFHQ
260
CVRLSKFDSE
270
RSISFIPPDG
280
EFELMRYRTT
290
KDIILPFRVI
300
PLVREVGRTK
310
LEVKVVIKSN
320
FKPSLLAQKI
330
EVRIPTPLNT
340
SGVQVICMKG
350
KAKYKASENA
360
IVWKIKRMAG
370
MKESQISAEI
380
ELLPTNDKKK
390
WARPPISMNF
400
EVPFAPSGLK
410
VRYLKVFEPK
420
LNYSDHDVIK
430
WVRYIGRSGI
YETRC
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0030121 |
AP-1 adaptor complex |
| Cellular Component |
GO:0030122 |
AP-2 adaptor complex |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0008021 |
synaptic vesicle |
| Molecular Function |
GO:0035615 |
clathrin adaptor activity |
| Molecular Function |
GO:0008289 |
lipid binding |
| Biological Process |
GO:0072583 |
clathrin-dependent endocytosis |
| Biological Process |
GO:0006896 |
Golgi to vacuole transport |
| Biological Process |
GO:0006886 |
intracellular protein transport |
| Biological Process |
GO:0031623 |
receptor internalization |
| Biological Process |
GO:0099003 |
vesicle-mediated transport in synapse |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.