Overview
| Uniprot ID | A0A286ZU42 |
|---|
| Protein Name | Phosphatidylinositol-binding clathrin assembly protein |
|---|
| Gene Name | PICALM |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 134 |
YSRYLNEKAVSYRQV |
| 147 |
QVAFDFTKVKRGADG |
| 231 |
DMKKNQCKEGLDIYK |
| 24 |
VTGSAVSKTVCKATT |
| 324 |
TKVDEREKQAALEEE |
Function
Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF)
Protein Sequence
10
MSGQSLTDRI
20
TAAQHSVTGS
30
AVSKTVCKAT
40
THEIMGPKKK
50
HLDYLIQCTN
60
EMNVNIPQLA
70
DSLFERTTNS
80
SWVVVFKSLI
90
TTHHLMVYGN
100
ERFIQYLASR
110
NTLFNLSNFL
120
DKSGLQGYDM
130
STFIRRYSRY
140
LNEKAVSYRQ
150
VAFDFTKVKR
160
GADGVMRTMN
170
TEKLLKTVPI
180
IQNQMDALLD
190
FNVNSNELTN
200
GVINAAFMLL
210
FKDAIRLFAA
220
YNEGIINLLE
230
KYFDMKKNQC
240
KEGLDIYKKF
250
LTRMTRISEF
260
LKVAEQVGID
270
RGDIPDLSQA
280
PSSLLDALEQ
290
HLASLEGKKI
300
KDSTAASRAT
310
TLSNAVSSLA
320
STGLSLTKVD
330
EREKQAALEE
340
EQARLKALKE
350
QRLKELAKKP
360
HTSLTTAASP
370
VSTSAGGIMT
380
APAIDIFSTP
390
SSSNSTSKLP
400
NDLLDLQQPT
410
FHPSVLPMSS
420
ASQVASTWGG
430
FTPSPVAQPH
440
PSAGLNVDFE
450
SVFGNKSTNV
460
IVDSGGFDEL
470
GGLLKPTVAS
480
QNQSLPVAKL
490
PPNKLVSDDL
500
DSSLANLVGN
510
LGIGNGTTKN
520
DVNWSQPGEK
530
KLTGGSNWQP
540
KVAPTTAWNA
550
ATMNGMHFPQ
560
YAPPVMAYPA
570
TTPTGMIGYG
580
IPPQMGSVPV
590
MTQPTLIYSQ
600
PVMRPPNPFG
610
PVSGAQIQFM
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005905 |
clathrin-coated pit |
| Cellular Component |
GO:0030136 |
clathrin-coated vesicle |
| Cellular Component |
GO:0005794 |
Golgi apparatus |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0005545 |
1-phosphatidylinositol binding |
| Molecular Function |
GO:0030276 |
clathrin binding |
| Biological Process |
GO:0048268 |
clathrin coat assembly |
| Biological Process |
GO:0072583 |
clathrin-dependent endocytosis |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.