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Overview

Uniprot ID	A0A286ZUW0
Protein Name	Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1
Gene Name	GFPT1
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
216	IGVRSEHKLSTDHIP
251	GERGKDKKGSCNLSR
379	GGLKDHIKEIQRCRR
640	RPVVICDKEDTETIK
647	KEDTETIKNTKRTIK

Function

Rate-limiting enzyme of the hexosamine biosynthetic pathway (HBP) that catalyzes the formation of glucosamine-6-phosphate from fructose-6-phosphate and glutamine, thereby controlling the flux of glucose into this pathway. Inhibited by UDP-N-acetylglucosamine (UDP-GlcNAc) through a feedback loop. Fine-tunes the metabolic fluctuations of UDP-GlcNAc and its impacts on hyaluronan synthesis during tissue remodeling. Via control of the HPB, regulates the availability of precursors for N- and O-linked protein glycosylation and modulates peripheral clock oscillation

Protein Sequence

10 FAGIFAYLNY 20 HVPRTRREIL 30 ETLIKGLQRL 40 EYRGYDSAGV 50 GVDGGNDKDW 60 EANACKIQLI 70 KKKGKVKALD 80 EEVHKQQDMD 90 LDIEFDVHLG 100 IAHTRWATHG 110 EPNPVNSHPQ 120 RSDKNNEFIV 130 IHNGIITNYK 140 DLKKFLESKG 150 YDFESETDTE 160 TIAKLVKYMY 170 DNRESQDTSF 180 TTLVERVIQQ 190 LEGAFALVFK 200 SVHFPGQAVG 210 TRRGSPLLIG 220 VRSEHKLSTD 230 HIPILYRTAR 240 TQIGSKFTRW 250 GSQGERGKDK 260 KGSCNLSRVD 270 STTCLFPVEE 280 KAVEYYFASD 290 ASAVIEHTNR 300 VIFLEDDDVA 310 AVVDGRLSIH 320 RIKRTAGDHP 330 GRAVQTLQME 340 LQQIMKGNFS 350 SFMQKEIFEQ 360 PESVVNTMRG 370 RVNFDDYTVN 380 LGGLKDHIKE 390 IQRCRRLILI 400 ACGTSYHAGV 410 ATRQVLEELT 420 ELPVMVELAS 430 DFLDRNTPVF 440 RDDVCFFISQ 450 SGETADTLMG 460 LRYCKERGAL 470 TVGITNTVGS 480 SISRETDCGV 490 HINAGPEIGV 500 ASTKAYTSQF 510 VSLVMFALMM 520 CDDRISMQER 530 RKEIMLGLKR 540 LPDLIKEVLS 550 MDDEIQKLAT 560 ELYHQKSVLI 570 MGRGYHYATC 580 LEGALKIKEI 590 TYMHSEGILA 600 GELKHGPLAL 610 VDKLMPVIMI 620 IMRDHTYAKC 630 QNALQQVVAR 640 QGRPVVICDK 650 EDTETIKNTK 660 RTIKVPHSVD 670 CLQGILSVIP 680 LQLLAFHLAV 690 LRGYDVSDGP 700 LGKSWAFDSF HLSF

Gene Ontology

Classification	GO ID	Description
Molecular Function	GO:0097367	carbohydrate derivative binding
Molecular Function	GO:0004360	glutamine-fructose-6-phosphate transaminase (isomerizing) activity
Biological Process	GO:0032922	circadian regulation of gene expression
Biological Process	GO:0006002	fructose 6-phosphate metabolic process
Biological Process	GO:0006048	UDP-N-acetylglucosamine biosynthetic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.