Overview
| Uniprot ID | A0A286ZVF7 |
|---|
| Protein Name | selenide, water dikinase |
|---|
| Gene Name | SEPHS1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 27 |
LTRFTELKGTGCKVP |
| 371 |
ARFCAEIKSPKYGEG |
Function
Core component of the zincore complex, a heterotetramer that acts as a molecular 'grip' to stabilize transcription factors at DNA-binding sites across the genome, thereby controlling gene expression. The zincore complex binds specifically to zinc finger transcription factors, such as ZFP91, ZNF652, ZNF526 and PRDM15, and stabilizes them onto their cognate DNA motif. Within the complex, SEPHS1, recognizes and binds the backbone of zinc fingers of transcription factors in a sequence-independent manner via its arginine clamp, enhancing their DNA-binding stability. Plays an essential role in redox homeostasis. May also be involved in selenocysteine biosynthesis by catalyzing formation of selenophosphate from selenide and ATP. Its role in selenocysteine biosynthesis is however unclear and several studies suggest that it does not act as a selenophosphate synthase in vivo or plays an non-essential role. Required for cardiac differentiation
Protein Sequence
10
MSARESFNPE
20
SYELDKSFRL
30
TRFTELKGTG
40
CKVPQDVLQK
50
LLESLQENHF
60
QEDEQFLGAV
70
MPRLAVATGL
80
RQSHSNAGSE
90
LRLQPTPQLT
100
AMLDRIGMDT
110
CVIPLRHGGL
120
SLVQTTDYIY
130
PIVDDPYMMG
140
RIACANVLSD
150
LYAMGVTECD
160
NMLMLLGISN
170
KMTDRERDKV
180
MPLIIQGFKD
190
AAEEAGTSVT
200
GGQTVLNPWI
210
VLGGVATTVC
220
QPNEFIMPDN
230
AVPGDVLVLT
240
KPLGTQVAVA
250
VHQWLDIPEK
260
WNKIKLVVTQ
270
EDVELAYQEA
280
MMNMARLNRT
290
AAGLMHTFNA
300
HAATDITGFG
310
ILGHAQNLAK
320
QQRNEVSFVI
330
HNLPVLAKMA
340
AVSKACGNMF
350
GLMHGTCPET
360
SGGLLICLPR
370
EQAARFCAEI
380
KSPKYGEGHQ
390
AWIIGIVEKG
400
NRTARIIDKP
410
RIIEVAPQVA
420
TQNVNPTPGA
TS
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0031965 |
nuclear membrane |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0004756 |
selenide, water dikinase activity |
| Biological Process |
GO:0045454 |
cell redox homeostasis |
| Biological Process |
GO:0016260 |
L-selenocysteine biosynthetic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.