Search Results

Overview

Uniprot ID	A0A286ZYB0
Protein Name	Malonate--CoA ligase ACSF3, mitochondrial
Gene Name	ACSF3
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
612	IIKSGGYKVSALEVE

Function

Catalyzes the initial reaction in intramitochondrial fatty acid synthesis, by activating malonate and methylmalonate, but not acetate, into their respective CoA thioester. May have some preference toward very-long-chain substrates

Protein Sequence

10 MGAVPRSAQQ 20 RVRAVGARSQ 30 SSAGRSAQWP 40 ARRARPATPG 50 LEPRGSAGLG 60 NVRARNPRRR 70 AAPRRGARLG 80 NGRRVRAPRS 90 GSGSDPARRR 100 RACPAGRGVP 110 SSLGVRLLPA 120 CAVTACPLPA 130 PGASCRLASA 140 MLPHVGRFPV 150 RCLLCALASC 160 RRAPWRPRGS 170 SALHTAPALR 180 SDGSGPVFTR 190 ALAFGDRIAL 200 VDQHGRHTYA 210 DLYSRSLRLS 220 REICALRACA 230 DGDLREERVS 240 LLCANDASYV 250 VALWATWMSG 260 GVAVPLYRKH 270 PPAQLEYFIR 280 DSRSSVVLAG 290 PEHAGVLGPV 300 VGKLGVPLLP 310 LSPEVYLGAA 320 EAPGAGRVPE 330 RDWRKRAATI 340 IYTSGTTGRP 350 KGVLSTHRNL 360 EAVVTGLVRK 370 WAWTQDDVIL 380 HVLPLHHVHG 390 LVNKLLCPLW 400 VGAACVMLPE 410 FSAQQVWEKF 420 LSPEAPRVNV 430 FMAVPTIYSK 440 LMDYHDRHFT 450 QPHVRDFVRA 460 VCEEKIRLMV 470 SGSAALPLPV 480 LEKWEGVTGH 490 TLLERYGMTE 500 IGMALSNSLT 510 TRLPGSVGTP 520 LPGVEVRIVS 530 GNLQKDGCPC 540 IVHAEGNERD 550 TEVTPGFEEK 560 EGELLVRGPS 570 VFREYWDQPE 580 ETRSAFTSDG 590 WFKTGDTAVF 600 RDSSYWIRGR 610 TSVDIIKSGG 620 YKVSALEVER 630 LLLGHPSITD 640 VAVIGVPDMT 650 WGQRVTAVVT 660 LQEGHSLSHR 670 ELKEWARGVL 680 APYAVPSELL 690 LVEEIPRNQM 700 GKVNKRELVR 710 QFYPGAPEAG RP

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0016405	CoA-ligase activity
Molecular Function	GO:0090409	malonyl-CoA synthetase activity
Molecular Function	GO:0031957	very long-chain fatty acid-CoA ligase activity
Biological Process	GO:0006633	fatty acid biosynthetic process
Biological Process	GO:0090410	malonate catabolic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.