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Overview

Uniprot IDA0A287A3Q5
Protein NameLysine--tRNA ligase
Gene NameKARS1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
223 KVDGAEPKLSKNELK
294 SQAVHQLKVSGEDPY
370 MANSRNYKSEEEFVR
627 FETEETRKILDDICV
636 LDDICVAKAVECPPP
685 LAKWHRSKEGLTERF
699 FELFVMKKEICNAYT

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity

Protein Sequence

10 MLGERALLHR 20 FPQGIAGDRA 30 GRAGMFLHVG 40 QDGHVVRVRE 50 ADPPRVRLRL 60 GLRGGGAASG 70 LPPGLRLWGL 80 VVRRSRAQPV 90 GLQLELLVAL 100 HAVQDVLRGD 110 EVARGLGRRL 120 PGLGQQHGPR 130 LLHPAQGAAA 140 VQDERREPAL 150 RRTGTHVEGH 160 GAAILPQEKS 170 RRMGRPIGVF 180 AQIHCLRHCE 190 RAAEELPPNE 200 RLAHGAPLPE 210 VRPSGKMAAV 220 QAAEVKVDGA 230 EPKLSKNELK 240 RRLKAEKKIA 250 EKEAKQKELS 260 EKQLSQATTA 270 ATNHTTDNGV 280 AAEEESLDPN 290 QYFKIRSQAV 300 HQLKVSGEDP 310 YPHKFHVDIS 320 LTHFIEEYSH 330 LQPGDHLTDI 340 TLKVAGRIHA 350 KRASGGKLIF 360 YDLRGEGVKL 370 QVMANSRNYK 380 SEEEFVRINN 390 KLRRGDIIGV 400 QGSPGKTKKG 410 ELSIIPYEIT 420 LLSPCLHMLP 430 HLHFGLKDKE 440 TRYRQRYLDL 450 ILNDFVRQKF 460 IIRSKIITYI 470 RSFLDALGFL 480 EIETPMMNII 490 PGGAVAKPFI 500 TYHNELDMNL 510 YMRIAPELYH 520 KMLVVGGIDR 530 VYEIGRQFRN 540 EGIDLTHNPE 550 FTTCEFYMAY 560 ADYHDLMEIT 570 EKMVSGMVKH 580 ITGSYKVTYH 590 PDGPEGQAYE 600 IDFTPPFRKI 610 SMVEELEKAL 620 GMKLPDTNLF 630 ETEETRKILD 640 DICVAKAVEC 650 PPPRTTARLL 660 DKLVGEFLEV 670 TCINPTFICD 680 HPQIMSPLAK 690 WHRSKEGLTE 700 RFELFVMKKE 710 ICNAYTELND 720 PVRQRQLFEE 730 QAKAKAAGDD 740 EAMFIDETFC 750 TALEYGLPPT 760 GGWGMGIDRV 770 TMFLTDSNNI 780 KEVLLFPAMK 790 PEDKKENVTA 800 TDTAENTAPG TSV

Gene Ontology

Classification GO ID Description
Cellular Component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005886 plasma membrane
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0003877 ATP:ADP adenylyltransferase activity
Molecular Function GO:0004824 lysine-tRNA ligase activity
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0000049 tRNA binding
Biological Process GO:0002276 basophil activation involved in immune response
Biological Process GO:0015966 diadenosine tetraphosphate biosynthetic process
Biological Process GO:0070371 ERK1 and ERK2 cascade
Biological Process GO:0006430 lysyl-tRNA aminoacylation
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0002863 positive regulation of inflammatory response to antigenic stimulus
Biological Process GO:0043032 positive regulation of macrophage activation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.