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Overview

Uniprot IDA0A287A3Z0
Protein NameMitotic spindle assembly checkpoint protein MAD1
Gene NameMAD1L1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
81 RAEQIRSKSHLIQVE

Function

Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling

Protein Sequence

10 MVRGKAPRDP 20 SLVFLQREVA 30 MEDLGENTTV 40 LSTLRSLNSF 50 ISQRVEGGSG 60 LDVSTPAQGS 70 LQMQYQQSMQ 80 LEERAEQIRS 90 KSHLIQVERE 100 KMQMELSHKR 110 ARVELERAAS 120 TSARSYEREV 130 DRNQELLTRI 140 RQLQEREAEA 150 EEKMKEQLER 160 YRLCQQSLDA 170 ASRKLREEED 180 SLAEAGETIS 190 ALKGRLSELQ 200 WSAMNQEMQV 210 KRLESEKQEL 220 KEQLDLQHKK 230 WQEAQQKVQE 240 LQASQDARAD 250 QEQRIKDLEQ 260 KLSLQEQDAA 270 IVRSMRSELV 280 RLPTMERELR 290 QLREENACLR 300 ETREASGLLR 310 EELEGLQRRL 320 GRQEKLQENL 330 VNLELENERL 340 LTKLQSWERL 350 GQTTGLSIRT 360 PEELSSFIVE 370 LQQRELTLKD 380 RNSTLTSSAR 390 GLEKARLQLQ 400 EEVRQVSSQL 410 LEERKKRETH 420 EALARRLQKR 430 VLLLTKERDG 440 MRAILGSYDS 450 ELTPAEYAPQ 460 LTRRLREAED 470 MVQKVHAHSS 480 EVEVQLSQAL 490 EELGGQKQRA 500 DALEMELQML 510 KSQAGPAEQS 520 FLFSREEVSA 530 LRLKIEELAQ 540 ERSRLEEDKK 550 LLEAQLERLT 560 LQGDFDQSRT 570 KVLHLSLNPA 580 SVARQRFRED 590 WQQLQEECKH 600 LRELVRALEA 610 GGPVPADLEA 620 AAGLPSSKEV 630 AELKRQVESA 640 ELKNQRLKEV 650 FQTKIQEFRK 660 ACYALTGYQV 670 DITTEGQFRL 680 TSMYAERKAD 690 CLVFKAAGPS 700 GTKMQLLETE 710 FSRTVPELIE 720 LHLLRQDSIP 730 AFLSALTLDL FSRQTVA

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005813 centrosome
Cellular Component GO:0000776 kinetochore
Cellular Component GO:1990706 MAD1 complex
Cellular Component GO:1990728 mitotic spindle assembly checkpoint MAD1-MAD2 complex
Cellular Component GO:0005635 nuclear envelope
Cellular Component GO:0000922 spindle pole
Biological Process GO:0051301 cell division
Biological Process GO:0007094 mitotic spindle assembly checkpoint signaling

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.