Overview
| Uniprot ID | A0A287A5N6 |
|---|
| Protein Name | Cleavage and polyadenylation specificity factor subunit 6 |
|---|
| Gene Name | CPSF6 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 146 |
KLMDLLPKRELHGQN |
| 496 |
ISLIKQSKVSADDRC |
Function
Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery. Plays a role in mRNA export
Protein Sequence
10
MADGVDHIDI
20
YADVGEEFNQ
30
EAEYGGHDQI
40
DLYDDVISPS
50
ANNGDAPEDR
60
DYMDTLPPTV
70
GDDVGKGAAP
80
NVVYTYTGKR
90
IALYIGNLTW
100
WTTDEDLTEA
110
VHSLGVNDIL
120
EIKFFENRAN
130
GQSKGFALVG
140
VGSEASSKKL
150
MDLLPKRELH
160
GQNPVVTPCN
170
KQFLSQFEMQ
180
SRKTTQSGQM
190
SGEGKAGPPG
200
GSSRAAFPQG
210
GRGRGRFPGA
220
VPGGDRFPGP
230
AGPGGPPPPF
240
PGNLIKHLVK
250
GTRPLFLETR
260
IPWHMGHSIE
270
EIPIFGLKAG
280
QTPPRPPLGP
290
PGPPGPPGPP
300
PPGQVLPPPL
310
AGPPNRGDRP
320
PPPVLFPGQP
330
FGQPPLGPLP
340
PGPPPPVPGY
350
GPPPGPPPPQ
360
QGPPPPPGPF
370
PPRPPGPLGP
380
PLTLAPPPHL
390
PGPPPGAPPP
400
APHVNPAFFP
410
PPTNSGMPTS
420
DSRGPPPTDP
430
YGRPPPYDRG
440
DYGPPGREMD
450
TARTPLSEAE
460
FEEIMNRNRA
470
ISSSAISRAV
480
SDASAGDYGS
490
AIETLVTAIS
500
LIKQSKVSAD
510
DRCKVLISSL
520
QDCLHGIESK
530
SYGSGSRRER
540
SRERDHSRSR
550
EKSRRHKSRS
560
RDRHDDYYRE
570
RSRERERHRD
580
RDRDRDRERD
REREYRHR
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0035061 |
interchromatin granule |
| Cellular Component |
GO:0005847 |
mRNA cleavage and polyadenylation specificity factor complex |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Cellular Component |
GO:0042382 |
paraspeckles |
| Cellular Component |
GO:0005726 |
perichromatin fibrils |
| Cellular Component |
GO:1990904 |
ribonucleoprotein complex |
| Molecular Function |
GO:1990448 |
exon-exon junction complex binding |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0043023 |
ribosomal large subunit binding |
| Biological Process |
GO:0180010 |
co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway |
| Biological Process |
GO:0110104 |
mRNA alternative polyadenylation |
| Biological Process |
GO:0046833 |
positive regulation of RNA export from nucleus |
| Biological Process |
GO:0051290 |
protein heterotetramerization |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.