Overview
| Uniprot ID | A0A287A7Q7 |
|---|
| Protein Name | Eukaryotic translation initiation factor 5 |
|---|
| Gene Name | EIF5 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 114 |
TDLHVNPKKQTIGNS |
| 115 |
DLHVNPKKQTIGNSC |
| 123 |
QTIGNSCKACGYRGM |
| 24 |
KMPRLIAKVEGKGNG |
| 364 |
ASKKYVSKELAKEIR |
| 368 |
YVSKELAKEIRVKAE |
| 412 |
SKTASVPKVEAVKSD |
| 95 |
MLDGFIKKFVLCPEC |
Function
Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC. When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP. Start codon recognition also induces a conformational change of the PIC to a closed state. This change increases the affinity of EIF5-CTD for EIF2-beta (EIF2S2), which allows the release, by an indirect mechanism, of EIF1 from the PIC. Finally, EIF5 stabilizes the PIC in its closed conformation
Protein Sequence
10
MSVNVNRSVS
20
DQFYRYKMPR
30
LIAKVEGKGN
40
GIKTVIVNMV
50
DVAKALNRPP
60
TYPTKYFGCE
70
LGAQTQFDVK
80
NDRYIVNGSH
90
EANKLQDMLD
100
GFIKKFVLCP
110
ECENPETDLH
120
VNPKKQTIGN
130
SCKACGYRGM
140
LDTHHKLCTF
150
ILKNPPENSD
160
SGTGKKEKEK
170
KNRKGKDKEN
180
GTVSSSETPP
190
PPPPNEINPP
200
PHAVEEEEDD
210
DWGEDTTEEA
220
QRRRMDEISD
230
HAKVLTLSDD
240
LERTVEERVN
250
ILFDFVKKKK
260
EEGIIDSSDK
270
EIVAEAERLD
280
VKAMGPLVLT
290
EVLFNEKIRE
300
QIKKYRRHFL
310
RFCHNNKKAQ
320
RYLLHGLECV
330
VAMHQAQLIS
340
KIPHILKEMY
350
DADLLEEEVI
360
ISWSEKASKK
370
YVSKELAKEI
380
RVKAEPFIKW
390
LKEAEEESSG
400
GEDDDEDENI
410
EVVYSKTASV
420
PKVEAVKSDN
430
KDDDIDIDAI
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0045202 |
synapse |
| Molecular Function |
GO:0071074 |
eukaryotic initiation factor eIF2 binding |
| Molecular Function |
GO:0005092 |
GDP-dissociation inhibitor activity |
| Molecular Function |
GO:0005525 |
GTP binding |
| Molecular Function |
GO:0005096 |
GTPase activator activity |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.