Overview
| Uniprot ID | A0A287A9Y2 |
|---|
| Protein Name | Serine/threonine-protein kinase RIO1 |
|---|
| Gene Name | RIOK1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 215 |
KTSILVFKDRDKYVS |
| 219 |
LVFKDRDKYVSGEFR |
Function
Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor. The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit. Despite the protein kinase domain is proposed to act predominantly as an ATPase. The catalytic activity regulates its dynamic association with the 40S subunit. In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation
Protein Sequence
10
MDYRRLLMSR
20
VVPGQFDDAD
30
SSDSEKIDSK
40
TVREDVLFED
50
LQNNVNEKMS
60
EGEMEEEEEE
70
EYDDDDWDWD
80
DGVGRLTRCY
90
ASSGGSNPQP
100
NRQTPNCNSA
110
RMSTPADKVL
120
RKFENKINLD
130
KLNVTDSVIN
140
KVTEKSRQKE
150
ADMYRIKDKA
160
DRATVEQVLD
170
PRTRMILFKM
180
LTRGVIAEIH
190
GCISTGKEAN
200
VYHARTASGE
210
SRAIKIYKTS
220
ILVFKDRDKY
230
VSGEFRFRHG
240
YCKGNPRKMV
250
KTWAEKEMRN
260
LIRLSTAQIP
270
CPEPVLLRSH
280
VLVMGFIGRG
290
DTPAPLLKNV
300
PLSESKAREL
310
YVQVIEYMRR
320
MYQDARLVHA
330
DLSEFNLLYQ
340
GGGVYVIDVS
350
QSVEHDHPHA
360
LEFLRKDCAN
370
VNDFFLKHGV
380
AVMTVRELFE
390
FVTDPSITHE
400
NVDAYLSKAM
410
EIASQRTKEE
420
KSSQEHVDEE
430
VFKRAYIPRT
440
LNDVKQYERD
450
VDVMMALKEE
460
DLALNAQQDS
470
ILYQTVTGLK
480
KDLSGVQKVP
490
ALLEDQVKEK
500
SCSDSEDTGS
510
SACSDTDSEE
520
RGGQANSRKP
530
TTDLEVDKKE
540
RKKMVKEAQR
550
EKRKNKIPKH
560
VKKRKEKTAK
TKKGK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0034708 |
methyltransferase complex |
| Cellular Component |
GO:0030688 |
preribosome, small subunit precursor |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016887 |
ATP hydrolysis activity |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0106310 |
protein serine kinase activity |
| Molecular Function |
GO:0004674 |
protein serine/threonine kinase activity |
| Biological Process |
GO:0030490 |
maturation of SSU-rRNA |
| Biological Process |
GO:2000234 |
positive regulation of rRNA processing |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.