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Overview

Uniprot IDA0A287ABU6
Protein NameDihydrofolate reductase
Gene NameDHFR
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
107 LTEQPELKNKVDMVW
33 PPLRNEYKYFQRMTT

Function

Catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydrofolate in a NADPH-dependent manner. Key enzyme in folate metabolism. Contributes to the nuclear and mitochondrial de novo thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2

Protein Sequence

10 MVRPLNCIVA 20 VSQNMGIGKN 30 GDLPWPPLRN 40 EYKYFQRMTT 50 TSSVEGKQNL 60 VIMGRKTWFS 70 IPEKNRPLKD 80 RINIVLSREL 90 KEPPQGAHFL 100 AKSLDDALKL 110 TEQPELKNKV 120 DMVWIVGGSS 130 VYKEAMNKPG 140 HIRLFVTRIM 150 KEFESDTFFP 160 EIDLEKYKLL 170 SECSGVPSDV 180 QEEKGIKYKF EVYEKNN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Biological Process GO:0031427 response to methotrexate
Biological Process GO:0006729 tetrahydrobiopterin biosynthetic process
Biological Process GO:0046654 tetrahydrofolate biosynthetic process
Biological Process GO:0035999 tetrahydrofolate interconversion
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005634 nucleus
Molecular Function GO:0004146 dihydrofolate reductase activity
Molecular Function GO:0033560 folate reductase activity
Molecular Function GO:0050661 NADP binding
Molecular Function GO:0003723 RNA binding
Biological Process GO:0046452 dihydrofolate metabolic process
Biological Process GO:0046655 folic acid metabolic process
Biological Process GO:2000121 regulation of removal of superoxide radicals

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.