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Overview

Uniprot IDA0A287ADU8
Protein NameATP-dependent clpX-like chaperone, mitochondrial
Gene NameCLPX
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
171 KPPPPPKKIYNYLDK

Function

ATP-dependent chaperone that functions as an unfoldase. As part of the ClpXP protease complex, it recognizes specific protein substrates, unfolds them using energy derived from ATP hydrolysis, and then translocates them to the proteolytic subunit (CLPP) of the ClpXP complex for degradation. Thanks to its chaperone activity, it also functions in the incorporation of the pyridoxal phosphate cofactor into 5-aminolevulinate synthase, thereby activating 5-aminolevulinate (ALA) synthesis, the first step in heme biosynthesis. This chaperone is also involved in the control of mtDNA nucleoid distribution, by regulating mitochondrial transcription factor A (TFAM) activity

Protein Sequence

10 MSGCGACTCG 20 ASAARLITSS 30 LASAQRGISC 40 GRIHIPVLGR 50 LGTFETQLLR 60 RVPFRTFTET 70 PAYFASKDGI 80 SKDGSGDGNK 90 KSASEGSSKK 100 SGSGNSGKGG 110 NQLRCPKCGD 120 LCTHVETFVS 130 STRFVKCEKC 140 HHFFVVLSEA 150 DSKKSIIKEP 160 ESAAEAVKLA 170 FQQKPPPPPK 180 KIYNYLDKYV 190 VGQSFAKKVL 200 SVAVYNHYKR 210 IYNNIPANLR 220 QQAEVEKQTS 230 LTPRELEIRR 240 REDEYRFTKL 250 LQIAGISPHG 260 NALGASMQQQ 270 VNQQIPQEKR 280 GGEVLDSSHD 290 DIKLEKSNIL 300 LLGPTGSGKT 310 LLAQTLAKCL 320 DVPFAICDCT 330 TLTQAGYVGE 340 DIESVIAKLL 350 QDANYNVEKA 360 QQGIVFLDEV 370 DKIGSVPGIH 380 QLRDVGGEGV 390 QQGLLKLLEG 400 TIVNVPEKNS 410 RKLRGETVQV 420 DTTNILFVAS 430 GAFNGLDRII 440 SRRKNEKYLG 450 FGTPSNLGKG 460 RRAAAAADLA 470 NRSGESNTHQ 480 DIEEKDRLLR 490 HVEARDLIEF 500 GMIPEFVGRL 510 PVVVPLHSLD 520 EKTLVQILTE 530 PRNAVIPQYQ 540 ALFSMDKCEL 550 NVTEDALKAI 560 ARLALERKTG 570 ARGLRSIMEK 580 LLLEPMFEVP 590 NSDIVCVEVD 600 KEVVEGKKEP 610 GYIRAPTKES 620 SEEEYDSGVE 630 EEGWPRQADA ANS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0009841 mitochondrial endopeptidase Clp complex
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0042645 mitochondrial nucleoid
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0004176 ATP-dependent peptidase activity
Molecular Function GO:0140662 ATP-dependent protein folding chaperone
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0016504 peptidase activator activity
Molecular Function GO:0051082 unfolded protein binding
Biological Process GO:0046034 ATP metabolic process
Biological Process GO:0051603 proteolysis involved in protein catabolic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.