Overview
| Uniprot ID | A0A287AGJ7 |
|---|
| Protein Name | Cleavage and polyadenylation specificity factor subunit 6 |
|---|
| Gene Name | CPSF6 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 146 |
KLMDLLPKRELHGQN |
| 459 |
ISLIKQSKVSADDRC |
Function
Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping, and hence activates directly the mRNA 3'-processing machinery. Plays a role in mRNA export
Protein Sequence
10
MADGVDHIDI
20
YADVGEEFNQ
30
EAEYGGHDQI
40
DLYDDVISPS
50
ANNGDAPEDR
60
DYMDTLPPTV
70
GDDVGKGAAP
80
NVVYTYTGKR
90
IALYIGNLTW
100
WTTDEDLTEA
110
VHSLGVNDIL
120
EIKFFENRAN
130
GQSKGFALVG
140
VGSEASSKKL
150
MDLLPKRELH
160
GQNPVVTPCN
170
KQFLSQFEMQ
180
SRKTTQSGQM
190
SGEGKAGPPG
200
GSSRAAFPQG
210
GRGRGRFPGA
220
VPGGDRFPGP
230
AGPGGPPPPF
240
PAGQTPPRPP
250
LGPPGPPGPP
260
GPPPPGQVLP
270
PPLAGPPNRG
280
DRPPPPVLFP
290
GQPFGQPPLG
300
PLPPGPPPPV
310
PGYGPPPGPP
320
PPQQGPPPPP
330
GPFPPRPPGP
340
LGPPLTLAPP
350
PHLPGPPPGA
360
PPPAPHVNPA
370
FFPPPTNSGM
380
PTSDSRGPPP
390
TDPYGRPPPY
400
DRGDYGPPGR
410
EMDTARTPLS
420
EAEFEEIMNR
430
NRAISSSAIS
440
RAVSDASAGD
450
YGSAIETLVT
460
AISLIKQSKV
470
SADDRCKVLI
480
SSLQDCLHGI
490
ESKSYGSGSR
500
RERSRERDHS
510
RSREKSRRHK
520
SRSRDRHDDY
530
YRERSRERER
540
HRDRDRDRDR
550
ERDREREYRH
R
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005847 |
mRNA cleavage and polyadenylation specificity factor complex |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Biological Process |
GO:0110104 |
mRNA alternative polyadenylation |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.