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Overview

Uniprot IDA0A287AH57
Protein NameUbiquitin-conjugating enzyme E2 J1
Gene NameUBE2J1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
8 METRYNLKSPAVKRL

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Functions in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum (ERAD) and is essential for cells to recover from ER stress. Plays a role in MAPKAPK2-dependent translational control of TNF synthesis. Also acts as a platform for perinuclear positioning of the endosomal system by mediating ubiquitination of SQSTM1 through interaction with the E3 ubiquitin-protein ligase RNF26. Plays a role in male fecundity through the interaction with the E3 ubiquitin-protein ligase RNF133

Protein Sequence

10 METRYNLKSP 20 AVKRLMKEAA 30 ELKDPTDHYH 40 AQPLEDNLFE 50 WHFTVRGPPD 60 SDFDGGVYHG 70 RIVLPPEYPM 80 KPPSIILLTA 90 NGRFEVGKKI 100 CLSISGHHPE 110 TWQPSWSIRT 120 ALLAIIGFMP 130 TKGEGAIGSL 140 DYTPEERRAL 150 AKKSQDFCCE 160 GCGFAMKDVL 170 LPLKSGSDSS 180 QADEEAKELA 190 RQISFKAEVN 200 SSGKTITESD 210 LNHSFSLHDL 220 QDDIPTTFQG 230 ATASTSYGVQ 240 NPSAASPEQP 250 AQPAAKNTSL 260 SPRQRRAQQQ 270 SQRRSSPSPD 280 VIQGQQPRDN 290 HTDHGGSAVL 300 IVILTLALAA 310 LIFRRIYLAN EYIFDFEI

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000836 Hrd1p ubiquitin ligase complex
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0061631 ubiquitin conjugating enzyme activity
Molecular Function GO:0031625 ubiquitin protein ligase binding
Biological Process GO:0036503 ERAD pathway
Biological Process GO:1904153 negative regulation of retrograde protein transport, ER to cytosol
Biological Process GO:0006487 protein N-linked glycosylation
Biological Process GO:0000209 protein polyubiquitination

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.