Overview
| Uniprot ID | A0A287AHC2 |
|---|
| Protein Name | Glucosamine-6-phosphate isomerase |
|---|
| Gene Name | GNPDA1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 71 |
TPLGCYKKLIEYYRN |
Function
Catalyzes the reversible conversion of alpha-D-glucosamine 6-phosphate (GlcN-6P) into beta-D-fructose 6-phosphate (Fru-6P) and ammonium ion, a regulatory reaction step in de novo uridine diphosphate-N-acetyl-alpha-D-glucosamine (UDP-GlcNAc) biosynthesis via hexosamine pathway. Deamination is coupled to aldo-keto isomerization mediating the metabolic flux from UDP-GlcNAc toward Fru-6P. At high ammonium level can drive amination and isomerization of Fru-6P toward hexosamines and UDP-GlcNAc synthesis. Has a role in fine tuning the metabolic fluctuations of cytosolic UDP-GlcNAc and their effects on hyaluronan synthesis that occur during tissue remodeling. Seems to trigger calcium oscillations in mammalian eggs. These oscillations serve as the essential trigger for egg activation and early development of the embryo
Protein Sequence
10
MWFTCNFSSN
20
THYREICQNK
30
MKLIILDHYS
40
QASEWAAKYI
50
RNRIIQFNPG
60
PDKYFTLGLP
70
TGSTPLGCYK
80
KLIEYYRNGD
90
LSFQYVKTFN
100
MDEYVGLPRD
110
HPESYHSFMW
120
NNFFKHIDIR
130
PENTHILDGN
140
AADLQAECDA
150
FEEKIQAAGG
160
VELFVGGIGP
170
DGHIAFNEPG
180
SSLVSRTRVK
190
TLAMDTILAN
200
ARFFDGDLAK
210
VPTMALTVGV
220
GTVMDAREVM
230
ILITGAHKAF
240
ALYKAIEEGV
250
SHMWTVSAFQ
260
QHPRTVFVCD
270
EDATLELKVK
280
TVKYFKGLML
290
VHNKLVDPLY
300
SIKEKETEKS
QSSKKPYSD
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Molecular Function |
GO:0004342 |
glucosamine-6-phosphate deaminase activity |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0016853 |
isomerase activity |
| Biological Process |
GO:0005975 |
carbohydrate metabolic process |
| Biological Process |
GO:0006091 |
generation of precursor metabolites and energy |
| Biological Process |
GO:0006043 |
glucosamine catabolic process |
| Biological Process |
GO:0006046 |
N-acetylglucosamine catabolic process |
| Biological Process |
GO:0019262 |
N-acetylneuraminate catabolic process |
| Biological Process |
GO:0006048 |
UDP-N-acetylglucosamine biosynthetic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.