Overview
| Uniprot ID | A0A287AL22 |
|---|
| Protein Name | Transcription initiation factor IIB |
|---|
| Gene Name | GTF2B |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 178 |
EGVPRTFKEICAVSR |
| 238 |
AATHIARKAVELDLV |
Function
General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Also exhibits autoacetyltransferase activity that contributes to the activated transcription
Protein Sequence
10
MASTSRLDAL
20
PRVTCPNHPD
30
AILVEDYRAG
40
DMICPECGLV
50
VGDRVIDVGS
60
EWRTFSNDKA
70
TKDPSRVGDS
80
QNPLLSDGDL
90
STMIGKGTGA
100
ASFDEFGNSK
110
YQNRRTMSSS
120
DRAMMNAFKE
130
ITTMADRINL
140
PRNIVDRTNN
150
LFKQVYEQKS
160
LKGRANDAIA
170
SACLYIACRQ
180
EGVPRTFKEI
190
CAVSRISKKE
200
IGRCFKLILK
210
ALETSVDLIT
220
TGDFMSRFCS
230
NLCLPKQVQM
240
AATHIARKAV
250
ELDLVPGRSP
260
ISVAAAAIYM
270
ASQASAEKRT
280
QKEIGDIAGV
290
ADVTIRQSYR
300
LIYPRAPDLF
310
PTDFKFDTPV
DKLPQL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0032153 |
cell division site |
| Cellular Component |
GO:0042585 |
germinal vesicle |
| Cellular Component |
GO:0000776 |
kinetochore |
| Cellular Component |
GO:0016604 |
nuclear body |
| Cellular Component |
GO:0032993 |
protein-DNA complex |
| Cellular Component |
GO:0005669 |
transcription factor TFIID complex |
| Molecular Function |
GO:0046966 |
nuclear thyroid hormone receptor binding |
| Molecular Function |
GO:1990841 |
promoter-specific chromatin binding |
| Molecular Function |
GO:0061733 |
protein-lysine-acetyltransferase activity |
| Molecular Function |
GO:0000993 |
RNA polymerase II complex binding |
| Molecular Function |
GO:0000979 |
RNA polymerase II core promoter sequence-specific DNA binding |
| Molecular Function |
GO:0016251 |
RNA polymerase II general transcription initiation factor activity |
| Molecular Function |
GO:0017025 |
TBP-class protein binding |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0051177 |
meiotic sister chromatid cohesion |
| Biological Process |
GO:1990114 |
RNA polymerase II core complex assembly |
| Biological Process |
GO:0051123 |
RNA polymerase II preinitiation complex assembly |
| Biological Process |
GO:0051225 |
spindle assembly |
| Biological Process |
GO:0001174 |
transcriptional start site selection at RNA polymerase II promoter |
| Biological Process |
GO:0019083 |
viral transcription |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.