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Overview

Uniprot ID	A0A287AM05
Protein Name	Mitochondrial import inner membrane translocase subunit TIM44
Gene Name	TIMM44
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
115	TGEVIRKKLGEITGT
151	EEAAKTAKQSAESVS

Function

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 to drive protein translocation into the matrix using ATP as an energy source

Protein Sequence

10 SKAGKSRRCF 20 SSGVHFLSRH 30 NLVPLPLGTY 40 QMPWPSGQQT 50 LSRSYSSGNR 60 KGFLSGLLDN 70 IKQELAKNKE 80 MKESIKKFRD 90 EARKLEESDA 100 LQEARRKYRT 110 IESETMRTGE 120 VIRKKLGEIT 130 GTVKESLDEV 140 SKSDLGRKIK 150 EGVEEAAKTA 160 KQSAESVSKG 170 GEKLGRTAAF 180 KALSQGVESV 190 KKEIDESVLG 200 QTGPYRRPER 210 LRKRKEFAGE 220 KFKEEKVFEA 230 NEEALGVVLH 240 KDSKWYQQWK 250 DFRDNNVVFN 260 RFFEMKMKYD 270 ESDNALIRAS 280 RVLTDKVTDL 290 LGGLFSKTEM 300 SEVLTEILRV 310 DPAFDKERFL 320 QQCENDIIPN 330 VLEAMISGEL 340 DILKDWCYEA 350 TYSQLAHPIQ 360 QAKALGLQFH 370 SRILDIDNID 380 LAMGKMMEQG 390 PVLIITFQAQ 400 LVMVIKNPKG 410 EVVEGDPVSK 420 AGREGRRQEQ 430 PDGSLWPPCT 440 DSRSESGFCD 450 SLGMAGWHLE 460 KSQGPSWTWW RLEGG

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005743	mitochondrial inner membrane
Molecular Function	GO:0005524	ATP binding
Biological Process	GO:0015031	protein transport

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.