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Overview

Uniprot IDA0A287AMA9
Protein NameATP-dependent RNA helicase DDX42
Gene NameDDX42
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
512 FKGGKGKKLNIGGGG

Function

ATP-dependent RNA helicase that binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Also mediates RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Required for assembly of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs: DDX42 associates transiently with the SF3B subcomplex of the 17S U2 SnRNP complex and is released after fulfilling its role in the assembly of 17S U2 SnRNP. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm

Protein Sequence

10 MELGMWNICR 20 EVCFSACFSR 30 GIRDDIEEED 40 DQEAYFRYMA 50 ENPTAGVVQE 60 EEEDNLEYDS 70 DGNPIAPSKK 80 IIDPLPPIDH 90 SEIDYPPFEK 100 NFYNEHEEIT 110 NLTPQQLIDL 120 RHKLNLRVSG 130 AAPPRPGSSF 140 AHFGFDEQLM 150 HQIRKSEYTQ 160 PTPIQCQGVP 170 VALSGRDMIG 180 IAKTGSGKTA 190 AFIWPMLIHI 200 MDQKELEPGD 210 GPIAVIVCPT 220 RELCQQIHAE 230 CKRFGKAYNL 240 RSVAVYGGGS 250 MWEQAKALQE 260 GAEIVVCTPG 270 RLIDHVKKKA 280 TNLQRVSYLV 290 FDEADRMFDM 300 GFGMILIFSI 310 ATFRKKIEKL 320 ARDILIDPIR 330 VVQGDIGEAN 340 EDVTQIVEIL 350 HSGPSKWNWL 360 TRRLVEFTSS 370 GSVLLFVTKK 380 ANAEELANNL 390 KQEGHNLGLL 400 HGDMDQSERN 410 KVISDFKKKD 420 IPVLVATDVA 430 ARGLDIPSIK 440 TVINYDVARD 450 IDTHTHRIGR 460 TGRAGEKGVA 470 YTLLTPKDSN 480 FAGDLVRNLE 490 GANQHVSKEL 500 LDLAMQNAWF 510 RKSRFKGGKG 520 KKLNIGGGGL 530 GYRERPGLGS 540 ENSDRGNNNN 550 VMSNYEAYKP 560 STGAMGDRLT 570 AMKAAFQSQY 580 KSHFVAASLS 590 NQKAGSSAAG 600 ASGWTSAGSL 610 NSVPTNSAQQ 620 GHNSPDSPIA 630 GATKGVPAFG 640 SAGNLSSAPV 650 TYPSAGAQGV 660 NNTAPGNNSR 670 EGTGGGNGKR 680 ERYTENRGGS 690 RHSHGESGNR 700 HGESPRHGDG 710 GRHGDGYRYP 720 ESSSRHADGR 730 RHTDGHPHGE 740 NRHGGGGGRH 750 GESRGASDGR 760 NGESRKESCN 770 RESKMDPKAD 780 STKADKTDSK 790 TDKTADGFAV 800 PEPPSARKVD 810 GTVKGYVLKC 820 ESIVFNFIFR KIFGN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016787 hydrolase activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.