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Overview

Uniprot IDA0A287ASN9
Protein NameATP-dependent RNA helicase DDX42
Gene NameDDX42
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
525 FKGGKGKKLNIGGGG

Function

ATP-dependent RNA helicase that binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Also mediates RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Required for assembly of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs: DDX42 associates transiently with the SF3B subcomplex of the 17S U2 SnRNP complex and is released after fulfilling its role in the assembly of 17S U2 SnRNP. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm

Protein Sequence

10 MELGMWNICR 20 EVCFSACFSR 30 GIRDDIEEED 40 DQEAYFRYMA 50 ENPTAGVVQE 60 EEEDNLEYDS 70 DGNPIAPSKK 80 IIDPLPPIDH 90 SEIDYPPFEK 100 NFYNEHEEIT 110 NLTPQQLIDL 120 RHKLNLRVSG 130 AAPPRPGSSF 140 AHFGFDEQLM 150 HQIRKSEYTQ 160 PTPIQCQGVP 170 VALSGRDMIG 180 IAKTGSGKTA 190 AFIWPMLIHI 200 MDQKELEPGD 210 GPIAVIVCPT 220 RELCQQIHAE 230 CKRFGKAYNL 240 RSVAVYGGGS 250 MWEQAKALQE 260 GAEIVVCTPG 270 RLIDHVKKKA 280 TNLQRVSYLV 290 FDEADRMFDM 300 GFEYQVRSIA 310 SHVRPDRQTL 320 LFSATFRKKI 330 EKLARDILID 340 PIRVVQGDIG 350 EANEDVTQIV 360 EILHSGPSKW 370 NWLTRRLVEF 380 TSSGSVLLFV 390 TKKANAEELA 400 NNLKQEGHNL 410 GLLHGDMDQS 420 ERNKVISDFK 430 KKDIPVLVAT 440 DVAARGLDIP 450 SIKTVINYDV 460 ARDIDTHTHR 470 IGRTGRAGEK 480 GVAYTLLTPK 490 DSNFAGDLVR 500 NLEGANQHVS 510 KELLDLAMQN 520 AWFRKSRFKG 530 GKGKKLNIGG 540 GGLGYRERPG 550 LGSENSDRGN 560 NNNVMSNYEA 570 YKPSTGAMGD 580 RLTAMKAAFQ 590 SQYKSHFVAA 600 SLSNQKAGSS 610 AAGASGWTSA 620 GSLNSVPTNS 630 AQQGHNSPDS 640 PIAGATKGVP 650 AFGSAGNLSS 660 APVTYPSAGA 670 QGVNNTAPGN 680 NSREGTGGGN 690 GKRERYTENR 700 GGSRHSHGES 710 GNRHGESPRH 720 GDGGRHGDGY 730 RYPESSSRHA 740 DGRRHTDGHP 750 HGENRHGGGG 760 GRHGESRGAS 770 DGRNGESRKE 780 SCNRESKMDP 790 KADSTKADKT 800 DSKTDKTADG 810 FAVPEPPSAR 820 KVDGTVKGYV 830 LKCESIVFNF IFRKIFGN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016787 hydrolase activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0003724 RNA helicase activity

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.