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Overview

Uniprot IDA0A287AU85
Protein NameDNA replication licensing factor MCM4
Gene NameMCM4
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
428 NPRVSNVKSVYKTHI
432 SNVKSVYKTHIDVIH
469 EKRVELLKELSRKPD
568 GLTAYVTKDPETRQL
781 AVDVEEAKRLHREAL

Function

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity

Protein Sequence

10 MSSPASTPSR 20 RSTRRAAPTQ 30 PGEYPGLGCA 40 PWGPRGGQAG 50 SAGSAPSPQS 60 PCGSGATCAD 70 AAMPFPLGQD 80 PPSAGEPQLL 90 PLSPGIELRS 100 SPRAPSAGAP 110 LDLDMSSPLT 120 YGTPSSRVEG 130 TPRSGVRGTP 140 VRQRPDLGSA 150 RKGLQVDLHS 160 DGPAAEDIVA 170 SEQSLGQKLV 180 IWGTDVNVAT 190 CKENFQRFIQ 200 RFIDPLAKEE 210 ENVGIDITEP 220 LYMQRLGEIN 230 ITGEPFLNVN 240 CEHIKSFDTN 250 LYRQLICYPQ 260 EVIPTFDMAV 270 NEIFFDRYPD 280 SILEHQIQVR 290 PFNALKTKNM 300 RNLNPEDIDQ 310 LIAISGMVIR 320 TSQLIPEMQE 330 AFFQCQVCAH 340 TARVEIDRGR 350 IAEPGTCERC 360 HTTHSMALIH 370 NRSVFSDKQM 380 IKLQESPEDM 390 PAGQTPHTVV 400 LFAHNDLVDK 410 VQPGDRVHVT 420 GIYRAVPIRI 430 NPRVSNVKSV 440 YKTHIDVIHY 450 RKTDSKRLHG 460 LDEEAEQKLF 470 SEKRVELLKE 480 LSRKPDIYER 490 LASALAPSIY 500 EHEDIKKGIL 510 LQLFGGTRKD 520 FSHTGRGKFR 530 AEINILLCGD 540 PGTSKSQLLQ 550 YVYNLVPRGQ 560 YTSGKGSSAV 570 GLTAYVTKDP 580 ETRQLVLQTG 590 ALVLSDNGIC 600 CIDEFDKMNE 610 STRSVLHEVM 620 EQQTLSIAKA 630 GIICQLNART 640 SILAAANPIE 650 SQWNPKKTTI 660 ENIQLPHTLL 670 SRFDLIFLML 680 DPQDEAYDRR 690 LAHHLVSLYY 700 QSEEQAQEEV 710 MDMAVLRDYI 720 AYAHSTVLPR 730 LSQDASQALI 740 EAYVDMRKIG 750 SSRGMVSAYP 760 RQLESLIRLA 770 EAHAKVRFSN 780 KVEAVDVEEA 790 KRLHREALKQ 800 SATDPRTGIV 810 DISILTTGMS 820 ATSRKRKEEL 830 AEALKKLILS 840 KGKTPALKYQ 850 QLFEDIRGQS 860 DIAITRDMFE 870 EALRALADDD 880 FLTVTGKTVR LL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005694 chromosome
Cellular Component GO:0042555 MCM complex
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0003678 DNA helicase activity
Molecular Function GO:0003697 single-stranded DNA binding
Biological Process GO:0006271 DNA strand elongation involved in DNA replication
Biological Process GO:0000727 double-strand break repair via break-induced replication
Biological Process GO:1902975 mitotic DNA replication initiation

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.