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Overview

Uniprot IDA0A287AWK9
Protein NameDCN1-like protein
Gene NameDCUN1D5
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
23 VAEDGSLKKCKISSY
8 MPVKKKRKSSGVAAA

Function

Contributes to the neddylation of all cullins by transferring NEDD8 from N-terminally acetylated NEDD8-conjugating E2s enzyme to different cullin C-terminal domain-RBX complexes which is necessary for the activation of cullin-RING E3 ubiquitin ligases (CRLs). May play a role in DNA damage response and may participate in cell proliferation and anchorage-independent cell growth

Protein Sequence

10 MPVKKKRKSS 20 GVAAAVAEDG 30 SLKKCKISSY 40 CRSQPPARLI 50 SGEEHFSSKK 60 CLAWFYEYAG 70 PDEVVGPEGM 80 EKFCEDIGVE 90 PENIIMLVLA 100 WKLEAESMGF 110 FTKEEWLKGM 120 TSLQCDCTEK 130 LQNKFDFLRS 140 QLNDISSFKN 150 IYRYAFDFAR 160 DKDQRSLDID 170 TAKSMLALLL 180 GRTWPLFSVF 190 YQYLEQSKYR 200 VMNKDQWYNV 210 LEFSRTVHAD 220 LSNYDEDGAW 230 PVLLDEFVEW QKVRQTS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005819 spindle
Cellular Component GO:0000151 ubiquitin ligase complex
Molecular Function GO:0097602 cullin family protein binding
Molecular Function GO:0031624 ubiquitin conjugating enzyme binding
Molecular Function GO:0032182 ubiquitin-like protein binding
Biological Process GO:0006974 DNA damage response
Biological Process GO:2000436 positive regulation of protein neddylation
Biological Process GO:0045116 protein neddylation
Biological Process GO:0001558 regulation of cell growth

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.