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Overview

Uniprot ID	A0A287AYH6
Protein Name	Transcription elongation factor SPT6
Gene Name	SUPT6H
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
634	VIKACSRKLYNWLRV

Function

Histone H3-H4 chaperone that plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A

Protein Sequence

10 MSDDEDDDEE 20 EYGKEEHEKE 30 AIAEEIFQDG 40 EGEEGQEAIE 50 APMAPPEEEE 60 EDDEESDIDD 70 FIVDDDGQPL 80 KKPKWRKKLP 90 GYTDAALQEA 100 QEIFGVDFDY 110 DEFEKYNEYD 120 EELEEEYEYE 130 DDEAEGEIRV 140 RPKKTTKKRV 150 SRRSIFEMYE 160 PSELESSHLT 170 DQDNEIRATD 180 LPERFQLRSI 190 PVKGAEDDEL 200 EEEADWIYRN 210 AFATPTISLQ 220 ESCDYLDRGQ 230 PTSSFSRKGP 240 STIQKIKEAL 250 GFMRNQHFEV 260 PFIAFYRKEY 270 VEPELHINDL 280 WRVWQWDEKW 290 TQLRIRKENL 300 TRLFEKMQAY 310 QYEQISADPD 320 KPLADGIRAL 330 DTTDMERLKD 340 VQSMDELKDV 350 YNHFLLYYGR 360 DIPKMQNAAK 370 ASRKKLKRVR 380 EEGDEEGDGE 390 EAEDEEQRGP 400 ELKQASRRDM 410 YTICQSAGLD 420 GLAKKFGLTP 430 EQFGENLRDS 440 YQRHETEQFP 450 AEPLELAKDY 460 VCSQFPTPEA 470 VLEGARYMVA 480 LQIAREPLVR 490 QVLRQTFQER 500 AKLNITPTKK 510 VVFELTPLPS 520 CLDVDEAHYA 530 YSFKYLKNKP 540 VKELRDDQFL 550 KICLAEDEGL 560 LTIDISIDMK 570 GVEGYGNDQT 580 YFEEIKQFYY 590 RDEFSHQVQE 600 WNRQRTMAIE 610 RALQQFLYVQ 620 MAKELKNKLL 630 AEAKEYVIKA 640 CSRKLYNWLR 650 VAPYRPDQQV 660 EEDDDFMDEN 670 QGKGIRVLGI 680 AFSSARDHPV 690 FCALVNGEGE 700 VTDFLRLPHF 710 TKRRTAWREE 720 EREKKAQDIE 730 TLKKFLLNKK 740 PHVVTVAGEN 750 RDAQMLIEDV 760 KRIVHELDQG 770 QQLSSIGVEL 780 IDNELAILYM 790 NSKKSEAEFR 800 DYPPVLRQAV 810 SLARRIQDPL 820 IEFAQVCSSD 830 EDILCLKFHP 840 LQEHVVKEEL 850 LNALYCEFIN 860 RVNEVGVDVN 870 RAIAHPYSQA 880 LIQYVCGLGP 890 RKGTHLLKIL 900 KQNNTRLESR 910 TQLVTMCHMG 920 PKVFMNCAGF 930 LKIDTASLGD 940 STDSYIEVLD 950 GSRVHPETYE 960 WARKMAVDAL 970 EYDESAEDAN 980 PAGALEEILE 990 NPERLKDLDL 1000 DAFAEELERQ 1010 GYGDKHITLY 1020 DIRAELSCRY 1030 KDLRTAYRSP 1040 NTEEIFNMLT 1050 KETPETFYIG 1060 KLIICNVTGI 1070 AHRRPQGESY 1080 DQAIRNDETG 1090 LWQCPFCQQD 1100 NFPELSEVWN 1110 HFDSGSCPGQ 1120 AIGVKTRLDN 1130 GVTGFIPTKF 1140 LSDKVVKRPE 1150 ERVKVGMTVH 1160 CRIMKIDIEK 1170 FSADLTCRTS 1180 DLMDRNNEWK 1190 LPKDTYYDFD 1200 AEAADHKQEE 1210 DMKRKQQRTT 1220 YIKRVIAHPS 1230 FHNINFKQAE 1240 KMMETMDQGD 1250 VIIRPSSKGE 1260 NHLTVTWKVS 1270 DGIYQHVDVR 1280 EEGKENAFSL 1290 GATLWINSEE 1300 FEDLDEIVAR 1310 YVQPMASFAR 1320 DLLNHKYYQD 1330 CSGGDRKKLE 1340 ELLIKTKKEK 1350 PTFIPYFICA 1360 CKELPGKFLL 1370 GYQPRGKPRI 1380 EYVTVTPEGF 1390 RYRGQIFPTV 1400 NGLFRWFKDH 1410 YQDPVPGITP 1420 SSSSRTRTPA 1430 SINATPANIN 1440 LADLTRAVNA 1450 LPQNMTSQMF 1460 SAIAAVTGQG 1470 QNPNATPAQW 1480 ASSQYGYGGS 1490 GGGSSAYHVF 1500 PTPAQQPVAT 1510 PLMTPSYSYT 1520 TPSQPITTPQ 1530 YHQLQASTTP 1540 QSAQAQPQPS 1550 SSSRQRQQQP 1560 KSNSHAAIDW 1570 GKMAEQWLQE 1580 KEAWLPIASL 1590 PAPPFLSIKW 1600 RELTFSLVVS LDG

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0003677	DNA binding
Biological Process	GO:0006397	mRNA processing
Biological Process	GO:0051028	mRNA transport
Biological Process	GO:0008380	RNA splicing
Biological Process	GO:0140673	transcription elongation-coupled chromatin remodeling

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.