Overview
| Uniprot ID | A0A287AZV4 |
|---|
| Protein Name | S-(hydroxymethyl)glutathione dehydrogenase |
|---|
| Gene Name | ADH5 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 125 |
CKFCLNPKTNLCQKI |
| 131 |
PKTNLCQKIRVTQGK |
| 244 |
IIGVDINKDKFARAK |
Function
Catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Also oxidizes long chain omega-hydroxy fatty acids, such as 20-HETE, producing both the intermediate aldehyde, 20-oxoarachidonate and the end product, a dicarboxylic acid, (5Z,8Z,11Z,14Z)-eicosatetraenedioate. Class-III ADH is remarkably ineffective in oxidizing ethanol. Required for clearance of cellular formaldehyde, a cytotoxic and carcinogenic metabolite that induces DNA damage. Also acts as a S-nitroso-glutathione reductase by catalyzing the NADH-dependent reduction of S-nitrosoglutathione, thereby regulating protein S-nitrosylation
Protein Sequence
10
MMNRILHCSE
20
EQTTRSLFSP
30
FQVIKCKAAV
40
AWEAGKPLSI
50
EEIEVAPPKA
60
HEVRIKIIAT
70
AVCHTDAYTL
80
SGADPEGSFP
90
VILGHEGAGI
100
VESVGEGVTK
110
LKAGDTVIPL
120
YIPQCGECKF
130
CLNPKTNLCQ
140
KIRVTQGKGL
150
MPDGTSRFTC
160
KGKTILHYMG
170
TSTFSEYTVV
180
ADISVAKIDP
190
LAPLDKVCLL
200
GCGVSTGYGA
210
AVNTAKVEPG
220
STCAVFGLGG
230
VGLAVIMGCK
240
VAGASRIIGV
250
DINKDKFARA
260
KEFGASECIN
270
PQDFSKPIQE
280
VLIEMTDGGV
290
DYSFECIGNV
300
KVMRAALEAC
310
HKGWGVSVVV
320
GVAASGEEIA
330
TRPFQLVTGR
340
TWKGTAFGGW
350
KSVESIPKLV
360
SEYMSKKIKV
370
DEFVTHNLPF
380
DQINEAFELM
390
HAGKSIRTVI
KL
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Molecular Function |
GO:0004022 |
alcohol dehydrogenase (NAD+) activity |
| Molecular Function |
GO:0106322 |
S-(hydroxymethyl)glutathione dehydrogenase (NAD+) activity |
| Molecular Function |
GO:0106321 |
S-(hydroxymethyl)glutathione dehydrogenase (NADP+) activity |
| Molecular Function |
GO:0051903 |
S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity |
| Molecular Function |
GO:0080007 |
S-nitrosoglutathione reductase (NADH) activity |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0046294 |
formaldehyde catabolic process |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.