Overview
| Uniprot ID | A0A287B8J2 |
|---|
| Protein Name | Dynactin subunit 1 |
|---|
| Gene Name | DCTN1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 618 |
LMPRLICKAELIRKQ |
| 77 |
DGTVQGRKYFTCDEG |
| 962 |
LKKSLKIKGEELSEA |
Function
Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules (PubMed:33734450). Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule. Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. Plays a role in metaphase spindle orientation. Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole. Plays a role in primary cilia formation (By similarity)
Protein Sequence
10
MAQSKRHVYS
20
RTPSGSRMSA
30
EASARPLRVG
40
SRVEVIGKGH
50
RGTVAYVGAT
60
LFATGKWVGV
70
ILDEAKGKND
80
GTVQGRKYFT
90
CDEGHGIFVR
100
QSQIQVFEDG
110
ADTTSPETPD
120
SSASKVLRRE
130
GTDSNAKTSK
140
LRGPKPKKAP
150
TARKTTTRRP
160
KPTRPASTGV
170
AGASSSLGPS
180
GSASAGELSS
190
SEPSTPAQTP
200
LAAPIIPTPA
210
LTSPGAAPPL
220
PSPSKEEEGL
230
RAQVRDLEEK
240
LETLRLKRAE
250
DKAKLKELEK
260
HKIQLEQVQE
270
WKSKMQEQQA
280
DLQRRLKEAR
290
KEAKEALEAK
300
ERYMEEMADT
310
ADAIEMATLD
320
KEMAEERAES
330
LQQEVEALKE
340
RVDELTTDLE
350
ILKAEIEEKG
360
SDGAASSYQL
370
KQLEEQNARL
380
KDALVRMRDL
390
SSSEKQEHVK
400
LQKLMEKKNQ
410
ELEVVRQQRE
420
RLQEELSQAE
430
STIDELKEQV
440
DAALGAEEMV
450
EMLTDRNLNL
460
EEKVRELRET
470
VGDLEAMNEM
480
NDELQENARE
490
TELELREQLD
500
MAGARVREAQ
510
KRVEAAQETV
520
ADYQQTIKKY
530
RQLTAHLQDV
540
NRELTNQQEA
550
SVERQQQPPP
560
ETFDFKIKFA
570
ETKAHAKAIE
580
MELRQMEVAQ
590
ANRHMSLLTA
600
FMPDSFLRPG
610
GDHDCVLVLL
620
LMPRLICKAE
630
LIRKQAQEKF
640
DLSENCSERP
650
GLRGAAGEQL
660
SFAAGLVYSL
670
SLLQATLHRY
680
EHALSQCSVD
690
VYKKVGSLYP
700
EMSAHERSLD
710
FLIELLHKDQ
720
LDETVNVEPL
730
TKAIKYYQHL
740
YSIHLAEQPE
750
DSTMQLADHI
760
KFTQSALDCM
770
SVEVGRLRAF
780
LQGGQEASDI
790
ALLLRDLETS
800
CSDIRQFCKK
810
IRRRMPGTDA
820
PGIPAALAFG
830
AQVSDTLLDC
840
RKHLTWVVAV
850
LQEVAAAAAQ
860
LIAPLAENEG
870
LPVAALEELA
880
FKASEQIYGT
890
PSSSPYECLR
900
QSCNILISTM
910
NKLATAMQEG
920
EYDAERPPSK
930
PPPVELRAAA
940
LRAEITDAEG
950
LGLKLEDRET
960
VIKELKKSLK
970
IKGEELSEAN
980
VRLSLLEKKL
990
DSAAKDADER
1000
IEKVQTRLEE
1010
TQALLRKKEK
1020
EFEETMDALQ
1030
ADIDQLEAEK
1040
AELKQRLNSQ
1050
SKRTIEGIRG
1060
PPPSGIATLV
1070
SGIAGEEQQR
1080
GGAPGQAPGI
1090
VPGPGLVKDS
1100
PLLLQQISAM
1110
RLHISQLQHE
1120
NSVLKGAQMK
1130
ASLAALPPLH
1140
VAKLSLPPHE
1150
GPGSELAAGA
1160
LYRKTNQLLE
1170
TLNQLSTHTH
1180
VVDITRSSPA
1190
AKSPSAQLLE
1200
QVTQLKSLSD
1210
TIEKLKDEVL
1220
KETVSQRPGA
1230
TVPTDFATFP
1240
SSAFLRAKEE
1250
QQDDTVYMGK
1260
VTFSCAAGLG
1270
QRHRLVLTQE
1280
QLHQLHDRLI
S
Gene Ontology
| Classification |
GO ID |
Description |
| Biological Process |
GO:0061744 |
motor behavior |
| Cellular Component |
GO:0030424 |
axon |
| Cellular Component |
GO:0005938 |
cell cortex |
| Cellular Component |
GO:0031252 |
cell leading edge |
| Cellular Component |
GO:0120103 |
centriolar subdistal appendage |
| Cellular Component |
GO:0005814 |
centriole |
| Cellular Component |
GO:0005813 |
centrosome |
| Cellular Component |
GO:0036064 |
ciliary basal body |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0030286 |
dynein complex |
| Cellular Component |
GO:0010008 |
endosome membrane |
| Cellular Component |
GO:0045171 |
intercellular bridge |
| Cellular Component |
GO:0000776 |
kinetochore |
| Cellular Component |
GO:0005875 |
microtubule associated complex |
| Cellular Component |
GO:0035371 |
microtubule plus-end |
| Cellular Component |
GO:0043025 |
neuronal cell body |
| Cellular Component |
GO:0005635 |
nuclear envelope |
| Cellular Component |
GO:0030904 |
retromer complex |
| Cellular Component |
GO:0000922 |
spindle pole |
| Molecular Function |
GO:0008017 |
microtubule binding |
| Molecular Function |
GO:0019901 |
protein kinase binding |
| Molecular Function |
GO:0032935 |
sterol sensor activity |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0010457 |
centriole-centriole cohesion |
| Biological Process |
GO:0042632 |
cholesterol homeostasis |
| Biological Process |
GO:0000132 |
establishment of mitotic spindle orientation |
| Biological Process |
GO:0090120 |
lysosome to ER cholesterol transport |
| Biological Process |
GO:0034454 |
microtubule anchoring at centrosome |
| Biological Process |
GO:0007017 |
microtubule-based process |
| Biological Process |
GO:0007077 |
mitotic nuclear membrane disassembly |
| Biological Process |
GO:0007528 |
neuromuscular junction development |
| Biological Process |
GO:0050905 |
neuromuscular process |
| Biological Process |
GO:0070050 |
neuron cellular homeostasis |
| Biological Process |
GO:1990535 |
neuron projection maintenance |
| Biological Process |
GO:1905515 |
non-motile cilium assembly |
| Biological Process |
GO:0007097 |
nuclear migration |
| Biological Process |
GO:0090063 |
positive regulation of microtubule nucleation |
| Biological Process |
GO:1904398 |
positive regulation of neuromuscular junction development |
| Biological Process |
GO:0060236 |
regulation of mitotic spindle organization |
| Biological Process |
GO:0042147 |
retrograde transport, endosome to Golgi |
| Biological Process |
GO:0021517 |
ventral spinal cord development |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.