Overview
| Uniprot ID | A0A287BIE8 |
|---|
| Protein Name | IST1 homolog |
|---|
| Gene Name | IST1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 165 |
VADQLCAKYSKEYGK |
| 168 |
QLCAKYSKEYGKLCR |
| 172 |
KYSKEYGKLCRTNQI |
Function
ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation. Is required for efficient abscission during cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST
Protein Sequence
10
MNECLSFYFP
20
NNKVAHSRLD
30
GMGLNLIRES
40
DPGYSRNTLF
50
SLVGWLSACR
60
PGPSILLLGD
70
WSGICALAEL
80
AQKARKEIAD
90
YLAAGKDERA
100
RIRVEHIIRE
110
DYLVEAMEIL
120
ELYCDLLLAR
130
FGLIQSMKEL
140
DSGLAESVST
150
LIWAAPRLQS
160
EVAELKIVAD
170
QLCAKYSKEY
180
GKLCRTNQIG
190
TVNDRLMHKL
200
SVEAPPKILV
210
ERYLIEIAKN
220
YNVPYEPDSV
230
VMAEAPPGVE
240
TDLIDVGFTD
250
DVKKGGSGRG
260
GGGGFTAPVG
270
GPDGTVPMPM
280
PMPMPSPSTP
290
FSYPLPKGPS
300
DFNGLPVGTY
310
QAFPNIHPPQ
320
IPATPPSYES
330
VDDINADKNV
340
SSQIVGPGPK
350
PEASAKTPSR
360
PVDTHDNFVL
370
PELPSVPDTL
380
PTASAGANTS
390
ASEDIDFDDL
400
SRRFEELKKK
T
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005813 |
centrosome |
| Molecular Function |
GO:0044877 |
protein-containing complex binding |
| Biological Process |
GO:0008104 |
intracellular protein localization |
| Biological Process |
GO:0061952 |
midbody abscission |
| Biological Process |
GO:0045862 |
positive regulation of proteolysis |
| Biological Process |
GO:0015031 |
protein transport |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0031410 |
cytoplasmic vesicle |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005793 |
endoplasmic reticulum-Golgi intermediate compartment |
| Cellular Component |
GO:0090543 |
Flemming body |
| Cellular Component |
GO:0005635 |
nuclear envelope |
| Molecular Function |
GO:0090541 |
MIT domain binding |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.