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Overview

Uniprot IDA0A287BJR7
Protein NameAP2-associated protein kinase 1
Gene NameAAK1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
169 VARLHQCKTPIIHRD
371 IAPRQRPKAGQTQPN

Function

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity

Protein Sequence

10 MKKFFDSRRE 20 QGGSGLGSGS 30 SGGGGSTSGL 40 GSGYIGRVFG 50 IGRQQVTVDE 60 VLAEGGFAIV 70 FLVRTSNGMK 80 CALKRMFVNN 90 EHDLQVCKRE 100 IQIMRDLSGH 110 KNIVGYIDSS 120 INNVSSGDVW 130 EVLILMDFCR 140 GGQVVNLMNQ 150 RLQTGFTESE 160 VLQIFCDTCE 170 AVARLHQCKT 180 PIIHRDLKVE 190 NILLHDRGHY 200 VLCDFGSATN 210 KFQNPQTEGV 220 NAVEDEIKKY 230 TTLSYRAPEM 240 VNLYSGKIIT 250 TKADIWALGC 260 LLYKLCYFTL 270 PFGESQVAIC 280 DGNFTIPDNS 290 RYSQDMHCLI 300 RYMLEPDPDK 310 RPDIYQVSFF 320 SFKLLKKECP 330 VPNVQNSPIP 340 AKLPEPVKAS 350 EAAAKKTQPK 360 ARLTDPIPTT 370 ETSIAPRQRP 380 KAGQTQPNPG 390 ILPIQPALTP 400 RKRAMVQPPP 410 QVAGSSNQPG 420 LLASVPQPKT 430 QAPPSQPLPQ 440 SQAKQPQAPP 450 APQQPPSAPA 460 QGLPAQAQAT 470 PQHQQQLFLK 480 QQPQPPQPQP 490 QAQAPPAAQQ 500 PSGTFYQQQQ 510 QQQQQQAQAQ 520 QFQAVHPAAQ 530 QPAIAQFPAV 540 SQGSSQQQLI 550 QNFYQQQQQQ 560 QQQQQLATTL 570 HQQQLLTQQA 580 ALQQKTTVAA 590 IQPPQAQPAT 600 ASQPPPAQEP 610 AIQAPVRQQP 620 KVQTTPPPAI 630 QGQKLGSLTP 640 PSSPKAQRAG 650 HRRILSDVTH 660 SAVFGVPASK 670 STQLLQAAAA 680 EASLNKSKSA 690 TTTPSGSPRA 700 SQQNVYNPSE 710 GSTWNPFDDD 720 NFSKLTAEEL 730 LNKDFAKLGE 740 GKHPEKLGGS 750 AESLIPGFQP 760 TQGDAFAASS 770 FAAGTAEKRK 780 GGQAVDSSLP 790 LLSVSDPFIP 800 LQVPDAPGLK 810 SPDTSLLLPD 820 LLPMTDPFGL 830 QREPNPCPVI 840 TIEHFKETAG 850 VKGLPLYPDP 860 SRGPGPKTQN 870 SLESDYLARD 880 GPSSNSSFHS 890 SEEEGTDLEG 900 DMLDCSGSRP 910 LLMESEEEDE 920 SCKPLQGKLG 930 GAVPFAQPEG 940 STEPAKAAQG 950 GRKNQFRALT 960 QPTADGLGEP 970 DVFATAPFRS 980 SRVPTDDTDI 990 FAKAPFVSKG 1000 SVALSQPEEA 1010 DVFLRAPFTK 1020 KKSLEELAGA 1030 QIATSQESPA 1040 QAGLLGPTDD 1050 VPLLPGLDRA 1060 VYPSVRAQYS 1070 VAGFAQQSNL 1080 PSHSVQAADR 1090 LDSISPRGPA 1100 LESGAHPNDR 1110 NKGPQPQKEV 1120 VSGPVAGKPF 1130 RPQSLSKYSR 1140 HYSPEDEPTP 1150 EAQPIAAYKI 1160 VSQTNKQSIA 1170 GSVSITSLSS 1180 RTTELPAADP 1190 FALAPFPSKS GKQKP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005905 clathrin-coated pit
Cellular Component GO:0098793 presynapse
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004674 protein serine/threonine kinase activity
Biological Process GO:0006897 endocytosis

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.