Overview
| Uniprot ID | A0A287BMV2 |
|---|
| Protein Name | Serine/arginine-rich splicing factor 1 |
|---|
| Gene Name | SRSF1 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 165 |
GVVEFVRKEDMTYAV |
| 179 |
VRKLDNTKFRSHEGE |
| 30 |
LPPDIRTKDIEDVFY |
| 48 |
AIRDIDLKNRRGGPP |
Function
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway
Protein Sequence
10
MSGGGVIRGP
20
AGNNDCRIYV
30
GNLPPDIRTK
40
DIEDVFYKYG
50
AIRDIDLKNR
60
RGGPPFAFVE
70
FEDPRDAEDA
80
VYGRDGYDYD
90
GYRLRVEFPR
100
SGRGTGRGGG
110
GGGGGGAPRG
120
RYGPPSRRSE
130
NRVVVSGLPP
140
SGSWQDLKDH
150
MREAGDVCYA
160
DVYRDGTGVV
170
EFVRKEDMTY
180
AVRKLDNTKF
190
RSHEGETAYI
200
RVKVDGPRSP
210
SYGRSRSRSR
220
SRSRSRSRSN
230
SRSRSYSPRR
240
SRGSPRYSPR
250
HSRSRSHISE
EMD
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0071013 |
catalytic step 2 spliceosome |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0035145 |
exon-exon junction complex |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Molecular Function |
GO:0044547 |
DNA topoisomerase binding |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0050733 |
RS domain binding |
| Biological Process |
GO:0000380 |
alternative mRNA splicing, via spliceosome |
| Biological Process |
GO:0097400 |
interleukin-17-mediated signaling pathway |
| Biological Process |
GO:0000395 |
mRNA 5'-splice site recognition |
| Biological Process |
GO:0048255 |
mRNA stabilization |
| Biological Process |
GO:0034504 |
protein localization to nucleus |
| Biological Process |
GO:0110012 |
protein localization to P-body |
| Biological Process |
GO:0043484 |
regulation of RNA splicing |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.