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Overview

Uniprot ID	A0A287BN32
Protein Name	Chromodomain-helicase-DNA-binding protein 8
Gene Name	CHD8
Organism	Sus scrofa

Kla Sites from experimental identification

Position	Flanking peptide
253	SKTKGKSKLNTITPV

Function

ATP-dependent chromatin-remodeling factor, it slides nucleosomes along DNA; nucleosome sliding requires ATP. Acts as a transcription repressor by remodeling chromatin structure and recruiting histone H1 to target genes. Suppresses p53/TP53-mediated apoptosis by recruiting histone H1 and preventing p53/TP53 transactivation activity. Acts as a negative regulator of Wnt signaling pathway by regulating beta-catenin (CTNNB1) activity. Negatively regulates CTNNB1-targeted gene expression by being recruited specifically to the promoter regions of several CTNNB1 responsive genes. Involved in both enhancer blocking and epigenetic remodeling at chromatin boundary via its interaction with CTCF. Acts as a suppressor of STAT3 activity by suppressing the LIF-induced STAT3 transcriptional activity. Also acts as a transcription activator via its interaction with ZNF143 by participating in efficient U6 RNA polymerase III transcription

Protein Sequence

10 MKGESKRITL 20 VLQQPQSGGP 30 QGHRHVVLGS 40 LPGKIVLQGN 50 QLAALTQAKN 60 AQGQPAKVVT 70 IQLQVQQPQQ 80 KIQIVPQPPS 90 SQPQPQQPPS 100 TQPVALSSVQ 110 QAQIMGPGQG 120 PGQRLSVPLK 130 VVLQPQAGSS 140 QGASSGLSVV 150 KVLSASEVAA 160 LSSPASSTPH 170 TGGKTGIEEN 180 RRLEHQKKQE 190 KANRIVAEAI 200 ARARARGEQN 210 IPRVLNEDEL 220 PSVRPEEEGE 230 KKRRKKSSGE 240 RLKEEKPKKS 250 KTSGTSKTKG 260 KSKLNTITPV 270 VGKKRKRNTS 280 SDNSDVEVMP 290 AQSPREDEES 300 SIQKRRSNRQ 310 VKRKKYTEDL 320 DIKITDDEEE 330 EEVDVTGPIK 340 TEPILPEPVQ 350 EPDGETLPSM 360 QFFVENPSEE 370 DAAIVDKVLS 380 MRIVKKELPS 390 GQYTEAEEFF 400 VKYKNYSYLH 410 CEWATISQLE 420 KDKRIHQKLK 430 RFKTKMAQMR 440 HFFHEDEEPF 450 NPDYVEVDRI 460 LDESHSIDKD 470 NGEPVIYYLV 480 KWCSLPYEDS 490 TWELKEDVDE 500 GKIREFKRIQ 510 SRHPELKRVN 520 RPQASAWKKL 530 ELSHEYKNRN 540 QLREYQLEGV 550 NWLLFNWYNR 560 QNCILADEMG 570 LGKTIQSIAF 580 LQEVYNVGIH 590 GPFLVIAPLS 600 TITNWEREFN 610 TWTEMNTIVY 620 HGSLASRQMI 630 QQYEMYCKDS 640 RGRLIPGAYK 650 FDALITTFEM 660 ILSDCPELRE 670 IEWRCVIIDE 680 AHRLKNRNCK 690 LLDSLKHMDL 700 EHKVLLTGTP 710 LQNTVEELFS 720 LLHFLEPSQF 730 PSESEFLKDF 740 GDLKTEEQVQ 750 KLQAILKPMM 760 LRRLKEDVEK 770 NLAPKQETII 780 EVELTNIQKK 790 YYRAILEKNF 800 SFLSKGAGHT 810 NMPNLLNTMM 820 ELRKCCNHPY 830 LINGAEEKIL 840 TEFREACHII 850 PHDFHLQAMV 860 RSAGKLVLID 870 KLLPKLKAGG 880 HKVLIFSQMV 890 RCLDILEDYL 900 IQRRYLYERI 910 DGRVRGNLRQ 920 AAIDRFSKPD 930 SDRFVFLLCT 940 RAGGLGINLT 950 AADTCIIFDS 960 DWNPQNDLQA 970 QARCHRIGQS 980 KAVKVYRLIT 990 RNSYEREMFD 1000 KASLKLGLDK 1010 AVLQSMSGRD 1020 GNITGIQQFS 1030 KKEIEDLLRK 1040 GAYAAIMEED 1050 DEGSKFCEED 1060 IDQILLRRTT 1070 TITIESEGKG 1080 STFAKASFVA 1090 SENRTDISLD 1100 DPNFWQKWAK 1110 KADLDMDLLN 1120 SKNNLVIDTP 1130 RVRKQTRHFS 1140 TLKDDDLVEF 1150 SDLESEDDER 1160 PRSRRHDRHH 1170 TYGRTDCFRV 1180 EKHLLVYGWG 1190 RWRDILSHGR 1200 FKRRMTERDV 1210 ETICRAILVY 1220 CLLHYRGDEN 1230 IKGFIWDLIS 1240 PAENGKTKEL 1250 QNHSGLSIPV 1260 PRGRKGKKVK 1270 SQSTFDIHKA 1280 DWIRKYNPDT 1290 LFQDESYKKH 1300 LKHQCNKVLL 1310 RVRMLYYLRQ 1320 EVIGDQAEKV 1330 LGGAIASEID 1340 IWFPVVDQLE 1350 VPTTWWDSEA 1360 DKSLLIGVFK 1370 HGYEKYNTMR 1380 ADPALCFLEK 1390 AGRPDDKAIA 1400 AEHRVLDNFS 1410 DIVEGVDFDK 1420 DCEDPEYKPL 1430 QGPPKDQDDE 1440 GDPLMMMDEE 1450 ISVIDGDEAQ 1460 VTQQPGHLFW 1470 PPGSALTARL 1480 RRLVTAYQRS 1490 YKREQMKIEA 1500 AERGDRRRRR 1510 CEAAFKLKEI 1520 ARREKQQRWT 1530 RREQTDFYRV 1540 VSTFGVEYDP 1550 DTMQFHWDRF 1560 RTFARLDKKT 1570 DESLTKYFHG 1580 FVAMCRQVCR 1590 LPPAAGDEPP 1600 DPNLFIEPIT 1610 EERASRTLYR 1620 IELLRRLREQ 1630 VLCHPLLEDR 1640 LALCQPPGPE 1650 LPKWWEPVRH 1660 DGELLRGAAR 1670 HGVSQTDCNI 1680 MQDPDFSFLA 1690 ARMNYMQNHQ 1700 AGAPAPSLSR 1710 CSTPLLHQQY 1720 TSRTASPLPL 1730 RPDAPVEKPP 1740 EETAAQVPSL 1750 ESLTLKLEHE 1760 VVARSRPTPQ 1770 DYEMRVAPSD 1780 TTPLVSRSVP 1790 PVKLEDEDDS 1800 DSELDLSKLS 1810 PSSSSSSSSS 1820 SSSSSTDESE 1830 DEKEEKLTDR 1840 SRSKLYDEES 1850 LLSLTMSQDG 1860 FPNEDGEQMT 1870 PELLLLQERQ 1880 RASEWPKDRV 1890 LINRIDLVCQ 1900 AVLSGKWPSS 1910 RRSQEMVTGG 1920 MLGPGNHLLD 1930 SPSLTPGEYG 1940 DSPVPTPRSS 1950 SAASMAEEEV 1960 SAVTTAAAQF 1970 TKLRRGMDEK 1980 EFTVQIKDEE 1990 GLKLTFQKHK 2000 LMANGVMGDG 2010 HPLFHKKKGN 2020 RKKLVELEVE 2030 CMEEPNHLDV 2040 DLETRIPVIN 2050 KVDGTLLVGE 2060 DAPRRAELEM 2070 WLQGHPEFAV 2080 DPRFLAYMED 2090 RRKQKWQRCK 2100 KNNKAELNCL 2110 GMEPVQTANS 2120 RNGKKGHHTE 2130 TVFNRVLPGP 2140 IAPDSSKKRA 2150 RRTRPDLSKM 2160 MALMQGGGTG 2170 SLSLHNTFQH 2180 SSSGLQSVSS 2190 LGHSSATSAS 2200 LPFMPFVMGG 2210 AASSPHVDSS 2220 TMLHHHHHHP 2230 HPHHHHHHHP 2240 GLRATGYPSS 2250 PATTTSGTAL 2260 RLPPLQPEED 2270 EDDEDEDDDD 2280 DLSQGYDSSE 2290 RDFSLIDDPM 2300 MPANSDSSED ADD

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0071339	MLL1 complex
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016887	ATP hydrolysis activity
Molecular Function	GO:0008013	beta-catenin binding
Molecular Function	GO:0003677	DNA binding
Molecular Function	GO:0003678	DNA helicase activity
Molecular Function	GO:0042393	histone binding
Molecular Function	GO:0002039	p53 binding
Biological Process	GO:0006338	chromatin remodeling
Biological Process	GO:0006351	DNA-templated transcription
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0045892	negative regulation of DNA-templated transcription
Biological Process	GO:0030178	negative regulation of Wnt signaling pathway
Biological Process	GO:0045893	positive regulation of DNA-templated transcription
Biological Process	GO:0016055	Wnt signaling pathway

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.