Overview
| Uniprot ID | A0A287BN69 |
|---|
| Protein Name | Spliceosome RNA helicase DDX39B |
|---|
| Gene Name | DDX39A |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 151 |
KDEEVLKKNCPHVVV |
| 176 |
RNRSLNLKNVKHFVL |
| 179 |
SLNLKNVKHFVLDEC |
Function
Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2; the effect of ALYREF/THOC4 is reported conflictingly
Protein Sequence
10
MAEQDVENEL
20
LDYEEDEEPQ
30
KDVKGSYVSI
40
HSSGFRDFLL
50
KPELLRAIVD
60
CGFEHPSEVQ
70
HECIPQAILG
80
MDVLCQAKSG
90
MGKTAVFVLA
100
TLQQIEPVNG
110
QVTVLVMCHT
120
RELAFQISKE
130
YERFSKYMPS
140
VKVSVFFGGL
150
SIKKDEEVLK
160
KNCPHVVVGT
170
PGRILALVRN
180
RSLNLKNVKH
190
FVLDECDKML
200
EQLDMRRDVQ
210
EIFRLTPHEK
220
QCMMFSATLS
230
KEIRPVCRKF
240
MQDPMEVFVD
250
DETKLTLHGL
260
QQYYVKLKDS
270
EKNRKLFDLL
280
DVLEFNQVVI
290
FVKSVQRCMA
300
LAQLLVEQNF
310
PAIAIHRGMA
320
QEERLSRYQQ
330
FKDFQRRILV
340
ATNLFGRGMD
350
IERVNIVFNY
360
DMPEDSDTYL
370
HRVARAGRFG
380
TKGLAITFVS
390
DENDAKILND
400
VQDRFEVNVA
410
ELPEEIDIST
420
YSKWLRQGWG
430
AMAVGPLSPP
440
HPLLTGLLVC
450
VLQLNRAGNH
MP
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0016607 |
nuclear speck |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0016787 |
hydrolase activity |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0003724 |
RNA helicase activity |
| Biological Process |
GO:0006406 |
mRNA export from nucleus |
| Biological Process |
GO:0000398 |
mRNA splicing, via spliceosome |
| Biological Process |
GO:0045824 |
negative regulation of innate immune response |
| Biological Process |
GO:0046832 |
negative regulation of RNA export from nucleus |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.