Overview
| Uniprot ID | A0A287BSF9 |
|---|
| Protein Name | RISC-loading complex subunit TARBP2 |
|---|
| Gene Name | TARBP2 |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 275 |
LRNSVGEKILSLRSC |
Function
Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto AGO2. AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds in vitro to the PRM1 3'-UTR. Seems to act as a repressor of translation. For some pre-miRNA substrates, may also alter the choice of cleavage site by DICER1. Negatively regulates IRF7-mediated IFN-beta signaling triggered by viral infection by inhibiting the phosphorylation of IRF7 and promoting its 'Lys'-48-linked ubiquitination and degradation
Protein Sequence
10
MSEEEQGSGT
20
TTGCGLPSIE
30
QMLAANPGKT
40
PISLLQEYGT
50
RIGKTPVYDL
60
LKAEGQAHQP
70
NFTFRVTVGD
80
TSCTGQGPSK
90
KAAKHKAAEV
100
ALKHLKGGSM
110
LEPALEDSSS
120
FSPLDSSLPE
130
DVPVFTTTAA
140
ATPIPSAVAT
150
RSPPMEVQPP
160
VSPQQSECNP
170
VGALQELVVQ
180
KGWRLPEYTV
190
TQESGPAHRK
200
EFTMTCRVER
210
FIEIGSGTSK
220
KLAKRNAAAK
230
MLLRVHTVPL
240
DARDGNEAEP
250
DDDHFSLGVG
260
SRLDGLRNRG
270
PGCTWDSLRN
280
SVGEKILSLR
290
SCSLGSLGAL
300
GPACCSVLSE
310
LSEEQAFHVS
320
YLDIEELSLS
330
GLCQCLVELS
340
TQPATVCHGS
350
ATTREAARGE
360
AARRALQYLK
IMAGSK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0016442 |
RISC complex |
| Cellular Component |
GO:0070578 |
RISC-loading complex |
| Molecular Function |
GO:0003725 |
double-stranded RNA binding |
| Molecular Function |
GO:0019899 |
enzyme binding |
| Molecular Function |
GO:0035198 |
miRNA binding |
| Molecular Function |
GO:0070883 |
pre-miRNA binding |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Molecular Function |
GO:0140311 |
protein sequestering activity |
| Molecular Function |
GO:0035197 |
siRNA binding |
| Biological Process |
GO:0098795 |
global gene silencing by mRNA cleavage |
| Biological Process |
GO:0039532 |
negative regulation of cytoplasmic pattern recognition receptor signaling pathway |
| Biological Process |
GO:0050689 |
negative regulation of defense response to virus by host |
| Biological Process |
GO:0045070 |
positive regulation of viral genome replication |
| Biological Process |
GO:0031054 |
pre-miRNA processing |
| Biological Process |
GO:1903798 |
regulation of miRNA processing |
| Biological Process |
GO:0070920 |
regulation of regulatory ncRNA processing |
| Biological Process |
GO:0070921 |
regulation of siRNA processing |
| Biological Process |
GO:0006417 |
regulation of translation |
| Biological Process |
GO:0046782 |
regulation of viral transcription |
| Biological Process |
GO:0070922 |
RISC complex assembly |
| Biological Process |
GO:0030422 |
siRNA processing |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.