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Overview

Uniprot IDA0A3B6QFG6
Protein NameSuccinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
Gene NameLOC123144854
OrganismTriticum aestivum

Kla Sites from experimental identification

Position Flanking peptide
119 LNQVLVTKQTGPQGK
179 VVDGLAPKTADRQSS
392 DAAEKAVKASVK***
59 VGSVQEVKEVLKNVF
70 KNVFPSEKEIVVKSQ

Function

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit

Protein Sequence

10 MVRGSLGRLA 20 SRTLSVAGKW 30 QHQQLRRLNI 40 HEYQGAELMG 50 KFGINVPKGA 60 VVGSVQEVKE 70 VLKNVFPSEK 80 EIVVKSQILA 90 GGRGLGTFKS 100 GLKGGVHIVK 110 AEEAESLAAK 120 MLNQVLVTKQ 130 TGPQGKVVGK 140 LIIACAEGGT 150 SIEDLAEKFP 160 DKIVKVPVDV 170 FKGITDEDAA 180 KVVDGLAPKT 190 ADRQSSIEQI 200 KKLYELFCKT 210 DCTMLEINPL 220 AETADKKLVA 230 ADAKLNFDDN 240 AAFRQKEIFA 250 LRDTTQEDPR 260 EVAAAKADLN 270 YIGLDGEIGC 280 MVNGAGLAMA 290 TMDIIKLHGG 300 TPANFLDVGG 310 SASEGQVVEA 320 FKILTSDDRV 330 KAILVNIFGG 340 IMKCDVIASG 350 IVNAAKQVDL 360 KVPVVVRLEG 370 TNVDQGKRIL 380 KESGMTLITA 390 EDLDDAAEKA VKASVK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0004775 succinate-CoA ligase (ADP-forming) activity
Biological Process GO:0006099 tricarboxylic acid cycle

Reference

[1] Zhu J, Guo W, Lan Y. Global Analysis of Lysine Lactylation of Germinated Seeds in Wheat.. Int J Mol Sci 24(22). 2023 Nov 11. PMID: 38003390.